Team:Groningen/Notebook/Wetwork 7July2012

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1.) pSac-CM isolated yield 200ng/ul
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2.) Gel run of gradient PCR. All primers can amplify their fragments in all temperatures: 150alsTF+Rev amplifies 150bp fragment, 250alsTF+Rev amplifies 250bp fragment, 300alsTF+Rev amplifies 300bp fragment, and 500alsTF+Rev amplifies 500bp fragment. Optimal annealing temperature: 58 degree celcius until 60 degree celcius, for all primer sets.
 
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3.) B.subtilis transformation yesterday was a success for pSac-CM. Both methods show transformants. For BBa_I742123: both methods show no transformant. This is not expected. Ask team Edinburgh?
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Renske
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1.) pSac-CM isolated yield 200ng/ul<br>
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Purified cDNA as [[Team:Groningen/Notebook/Wetwork_5July2012|previously]]. Concentrations far too low (22.1 ng/uL). RNA degraded? Wrong handling? --> Ask Arjan to repeat work on Monday.
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2.) Gel run of gradient PCR. All primers can amplify their fragments in all temperatures: 150alsTF+Rev amplifies 150bp fragment, 250alsTF+Rev amplifies 250bp fragment, 300alsTF+Rev amplifies 300bp fragment, and 500alsTF+Rev amplifies 500bp fragment. Optimal annealing temperature: 58 degree celcius until 60 degree celcius, for all primer sets.<br>
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3.) B.subtilis transformation yesterday was a success for pSac-CM. Both methods show transformants. For BBa_I742123: both methods show no transformant. This is not expected. Ask team Edinburgh?<br>
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Renske<br>
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Purified cDNA as [[Team:Groningen/Notebook/Wetwork_5July2012|previously]]. Concentrations far too low (22.1 ng/uL). RNA degraded? Wrong handling? --> Ask Arjan to repeat work on Monday.<br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 17:01, 25 September 2012







Emeraldo
1.) pSac-CM isolated yield 200ng/ul
2.) Gel run of gradient PCR. All primers can amplify their fragments in all temperatures: 150alsTF+Rev amplifies 150bp fragment, 250alsTF+Rev amplifies 250bp fragment, 300alsTF+Rev amplifies 300bp fragment, and 500alsTF+Rev amplifies 500bp fragment. Optimal annealing temperature: 58 degree celcius until 60 degree celcius, for all primer sets.
3.) B.subtilis transformation yesterday was a success for pSac-CM. Both methods show transformants. For BBa_I742123: both methods show no transformant. This is not expected. Ask team Edinburgh?

Renske
Purified cDNA as [[Team:Groningen/Notebook/Wetwork_5July2012|previously]]. Concentrations far too low (22.1 ng/uL). RNA degraded? Wrong handling? --> Ask Arjan to repeat work on Monday.

Back to notebook

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