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Team:Fudan Lux/Notebook
From 2012.igem.org
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| - | <p>Discuss the establishment of the gene circuits.<img src="https://static.igem.org/mediawiki/2012/6/64/Gene_circuits1.jpg" style="float:right;width:300px;"alt="test"></p> | + | <p>Discuss the establishment of the gene circuits.</p> |
| + | <img src="https://static.igem.org/mediawiki/2012/6/64/Gene_circuits1.jpg" style="float:right;width:300px;"alt="test"> | ||
| + | <p>Get lux without operon and promoter from k325909 by PCR. | ||
| + | We used longPCR Enzyme and Overlapping PCR. | ||
| + | </p><br> | ||
| + | <p>Measure the growth curve of dH5a transformed by lux with araBAD (k325909)</p> | ||
| + | <img src="http://partsregistry.org/wiki/images/4/4c/Growth_curve_of_K325909.jpg" style="width:300px;"alt="test"><br> | ||
| + | <p>Transform tetR (<a href="http://partsregistry.org/Part:BBa_C0040">C0040</a>) from the kit. Transfer araBAD (<a href=" http://partsregistry.org/Part:BBa_I0500">I0500</a>) and tetR (<a href="http://partsregistry.org/Part:BBa_C0040">C0040</a>) with RBS into pSB1C3 by 3A ligation. Transform it into dH5a.</p><br><p>Transfer tetR with promoter and RFP with ptetO as promoter (<a href="http://partsregistry.org/Part:BBa_J61002">J61002</a>) into pSB1C3 by 3A ligation. Transform it into dH5a.</p><br> | ||
| + | <p>Sorry to find the ligation is failed. But fortunately, we decide to change the reporter into GFP (<a href="http://partsregistry.org/Part:BBa_I13522">I13522</a>). Transform the plasmid from the kit into dH5a.</p><br> | ||
| + | <p>Design protein composed by lov domain and modified helix turn helix (abbr. lov-HTH).</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2012/f/fd/Lov_demestration.jpg" style="width:300px;"alt="test"><br> | ||
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Revision as of 14:16, 25 September 2012
We summarized our recent brain storm result. At last we chose to make a light-signal oscillator 
in E.coli and possibilities to use nanotubes.
The first thing for the next week is to find an efficient light sensor and a stable lighting part.
Discuss the establishment of the gene circuits.
Get lux without operon and promoter from k325909 by PCR. We used longPCR Enzyme and Overlapping PCR.
Measure the growth curve of dH5a transformed by lux with araBAD (k325909)
Transform tetR (C0040) from the kit. Transfer araBAD (I0500) and tetR (C0040) with RBS into pSB1C3 by 3A ligation. Transform it into dH5a.
Transfer tetR with promoter and RFP with ptetO as promoter (J61002) into pSB1C3 by 3A ligation. Transform it into dH5a.
Sorry to find the ligation is failed. But fortunately, we decide to change the reporter into GFP (I13522). Transform the plasmid from the kit into dH5a.
Design protein composed by lov domain and modified helix turn helix (abbr. lov-HTH).
- Definition list
- Consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
- Lorem ipsum dolor sit amet
- Consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, commodo vitae, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. Donec non enim in turpis pulvinar facilisis. Ut felis. Praesent dapibus, neque id cursus faucibus, tortor neque egestas augue, eu vulputate magna eros eu erat. Aliquam erat volutpat. Nam dui mi, tincidunt quis, accumsan porttitor, facilisis luctus, metus
Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, commodo vitae, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. Donec non enim in turpis pulvinar facilisis. Ut felis. Praesent dapibus, neque id cursus faucibus, tortor neque egestas augue, eu vulputate magna eros eu erat. Aliquam erat volutpat. Nam dui mi, tincidunt quis, accumsan porttitor, facilisis luctus, metus
Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, commodo vitae, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. Donec non enim in turpis pulvinar facilisis. Ut felis. Praesent dapibus, neque id cursus faucibus, tortor neque egestas augue, eu vulputate magna eros eu erat. Aliquam erat volutpat. Nam dui mi, tincidunt quis, accumsan porttitor, facilisis luctus, metus
Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, commodo vitae, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt cond
