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Team:Groningen/Notebook/Wetwork 14August2012
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alsT promoter PCR -->OK! Proceeding to ligation and transformation tomorrow. | alsT promoter PCR -->OK! Proceeding to ligation and transformation tomorrow. | ||
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| + | Another terminator+biobrick suffix and prefix PCR, cut the purified PCR product and pSac-Cm with EcoRI and HindIII, ligate both cut product and then transform E. coli with the ligated product. | ||
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
Revision as of 13:57, 25 September 2012
Tom
Ligation of Fnr promotor + GFP in psb1c3. Ratio of ligation 1:1:1 and 2:1:1 Fnr:GFP:psb1c3.
Result: Transformation in E.coli did not work.
Nisa
alsT promoter PCR -->OK! Proceeding to ligation and transformation tomorrow.
Emeraldo
Another terminator+biobrick suffix and prefix PCR, cut the purified PCR product and pSac-Cm with EcoRI and HindIII, ligate both cut product and then transform E. coli with the ligated product. Back to notebook
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