Team:Groningen/Construct

From 2012.igem.org

(Difference between revisions)
Line 162: Line 162:
</html>
</html>
<html>
<html>
-
<div style="position:absolute; left: 200px; top: 400px; z-index:1;">
+
<div style="position:absolute; left: 200px; top: 350px; z-index:1;">
<img src="https://static.igem.org/mediawiki/2012/1/1c/Groningen2012_JP_20120618_pRe-CII-Lycopene_Construct_A.png" width="640" >
<img src="https://static.igem.org/mediawiki/2012/1/1c/Groningen2012_JP_20120618_pRe-CII-Lycopene_Construct_A.png" width="640" >
</div>
</div>
-
<div style="position:absolute; left: 200px; top: 620px; z-index:1;">
+
<div style="position:absolute; left: 200px; top: 570px; z-index:1;">
<img src="https://static.igem.org/mediawiki/2012/7/7e/Groningen2012_JP_20120618_pRe-CII-Violacein_Construct_A.png" width="640" >
<img src="https://static.igem.org/mediawiki/2012/7/7e/Groningen2012_JP_20120618_pRe-CII-Violacein_Construct_A.png" width="640" >
</div>
</div>

Revision as of 17:06, 23 September 2012




Construct


The standard construct is a combination of biobricks.

  1. Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
  2. BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
  3. BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.










  4. Or, using BBa_K274002 (Reporter) - Violet pigment producer.


Our sponsors:

Retrieved from "http://2012.igem.org/Team:Groningen/Construct"