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Team:Groningen/Modeling
From 2012.igem.org
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* Concentration and production rates as a function of temperature, volume, type of meat, geometry. | * Concentration and production rates as a function of temperature, volume, type of meat, geometry. | ||
'''''Life Support''''' | '''''Life Support''''' | ||
| - | # How | + | # How much glutamine is required to keep GS active till there is extracellular ammonium available? |
# What nutrients are required to support growth? | # What nutrients are required to support growth? | ||
# What is the growth rate (doubling time) vs. temperature using the minimum media? | # What is the growth rate (doubling time) vs. temperature using the minimum media? | ||
Revision as of 18:53, 19 June 2012
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| Fig. m2. Reactions involved between ammonium uptake and TnrA. |
References
- SubtiWiki, http://subtiwiki.uni-goettingen.de/wiki/index.php
- K. Gunka, F.M. Commichau, “Control of glutamate homeostasis in Bacillus subtilis: a complex interplay between ammonium assimilation, glutamate biosynthesis and degradation,” Molecular Biology, under review (2012).
- N.A. Doroshchuk, M.S. Gelfand, D.A. Rodlanov, “Regulation of Nitrogen Metabolism in Gram-Positive Bacteria,” Molecular Biology, vol. 40(5), pp. 829-836, (2006).
- Study Guide, Chem153C, University of California, Los Angeles. http://vohweb.chem.ucla.edu/voh/classes%5Cspring10%5C153CID28%5C11AminoAcidBiosynthesisSQA.pdf
Germination
- Effects of h2o and L-alanine concentrations on germination rate.
Volatile production
- Concentration and production rates as a function of temperature, volume, type of meat, geometry.
Life Support
- How much glutamine is required to keep GS active till there is extracellular ammonium available?
- What nutrients are required to support growth?
- What is the growth rate (doubling time) vs. temperature using the minimum media?
- What metabolites are required for lycopene production?
- What are the rates of consumption for various temperatures as the biomass grows?
- What is the total amount of nutrients required to support X amount of biomass?
Signaling Functionality
- What will the cell do in the presence of large concentration of ammonium?
- Is TnrA a function of glutamine concentration, or the rate of NH4 conversion into glutamine?
- Opacity as a function of lycopene concentration
