Revision as of 05:06, 23 September 2012

UNAM-Genomics_Mexico

Arabinose/Xylose AND Gate

Nanotubes!!

The logic

Random info

1. 1 kb ladder
2.E1010

06/07/12

PY BROTH 10g salt/L
PSB2K3 kanamicin
We transformed RFP E1010.
plate 1 18F
plate 2 17E
Stock 50 mg/mL
E.coli 1/1000
TRANSFORMATION PROTOCOL.
We made liquid cultures with colonies LIQUID CULTURE.
We extracted plasmids PLASMID EXTRACTION PROTOCOL.
We ran a gel to check the extraction GEL ELECTROPHORESIS PROTOCOL.

06/12/12

We made glycerols of the bacteria
GLYCEROL PROTOCOL .
We transformed plasmid pHp45 TRANSFORMATION PROTOCOL.

1. 1 kb ladder
2.E1010

06/13/12

We ran gel and extracted from gel LIQUID CULTURE.
We made liquid cultures LIQUID CULTURE and lysed [LYSIS PROTOCOL].

1. 1 kb ladder
We extracted from gel

06/14/12

We extracted from gel LIQUID CULTURE.
We made glycerols of pHp45 Ω GLYCEROL PROTOCOL .
We transformed PSB2K3 Km (Kanamycin) 5C BBa_J04450 PSB4A5 Am (ampicillin) 1I BBa_J04450 AraC BBa_C0080
2012 14L plate 1 pSB2K3 Km+
2011 14L plate 1 pSB2K3 Km+
2012 14L plate 1 pSB2K3 Km+
TRANSFORMATION PROTOCOL.

1. 1 kb ladder
pHp45 Ω with E/P
We extracted from gel

06/15/12

> Digested pHp45 Ω with E/P LIQUID CULTURE.
> We extracted plasmid with kit.
> Ran a gel GEL ELECTROPHORESIS PROTOCOL . (5)
>Extracted from gel LIQUID CULTURE.

ARAC
>From the overnight plate we prepared liquid culture LIQUID CULTURE.
>We left them incubating overnight.
>We left a plate (LB Km DH5α C0080 and a control).

06/18/12

>Plasmid extraction AraC with kit.
>C0080-psB2K3 915 bp
>Streaked 4 LB Km 30 plates DH5α psB2K3.
>It seemed as though there were two different colonies (white) and there had been a mutation. Normally they are red.
>Ran gel with Spr/Strr (eppendorf -20ºC) GEL ELECTROPHORESIS PROTOCOL.
>Made glycerols from one tube of DH5α C0080 Km 50 -> 04.
>Made glycerols from one tube of DH5α PSB4A5 Amp 100 -> 05 GLYCEROL PROTOCOL.
>Digested C0080 X,S.
>Digested B0014 E,P LIQUID CULTURE.

@@ Line 124: / Line 124: @@
 <li>
 <img src='https://static.igem.org/mediawiki/2012/5/56/UnamgenomicsandsugarBitacora_5.jpg' height="300"/>
-<div class='captionrojo'>
+<div class='captionverde'>
 <p class='captionInside'>1. 1 kb ladder <br />
 pHp45 Ω with E/P<br />
@@ Line 138: / Line 138: @@
 > Ran a gel [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_electrophoresis | GEL ELECTROPHORESIS PROTOCOL]] . (5) <br />
 >Extracted from gel [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_extraction_protocol | LIQUID CULTURE]]. <br />
+<br />
 ARAC<br />
 >From the overnight plate we prepared liquid culture [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Liquid_culture | LIQUID CULTURE]]. <br />
 >We left them incubating overnight. <br />
->We left a plate (LB Km DH5 C0080 and a control). <br />
+>We left a plate (LB Km DH5α C0080 and a control). <br />
+<br />
+<br />
+<br />
+<br />
+<br />
+<br />
+<h2>06/18/12</h2><br />
+>Plasmid extraction AraC with kit. <br />
+>C0080-psB2K3 915 bp<br />
+>Streaked 4 LB Km 30 plates DH5α psB2K3. <br />
+>It seemed as though there were two different colonies (white) and there had been a mutation. Normally they are red. <br />
+>Ran gel with Spr/Strr (eppendorf -20ºC) [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_electrophoresis | GEL ELECTROPHORESIS PROTOCOL]]. <br />
+>Made glycerols from one tube of DH5α C0080 Km 50 -> 04. <br />
+>Made glycerols from one tube of DH5α PSB4A5 Amp 100 -> 05 [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#GLYCEROL_PROTOCOL | GLYCEROL PROTOCOL]]. <br />
+>Digested C0080 X,S. <br />
+>Digested B0014 E,P [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Digestion_protocol_.2820_.C2.B5l.29 | LIQUID CULTURE]]. <br />
 }}

Team:UNAM Genomics Mexico/Notebook/ANDSugar

From 2012.igem.org