Team:Goettingen/week4-3

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Revision as of 10:50, 22 September 2012


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Preparation of plates, solutions

Experiment:
Preparation of 0.1 M IPTG solution. Preparation of LB-Amp-Xgal/IPTG agar plates.

Amplifcation of the tar gene

Experiment:
PCR was performed with isolated genomic DNA from DH10B, forward and reverse primers of tar and using Pfu polymerase. Clean up with peqGOLD Gelextraction Kit (Peqlab). A band at about 1.6 kb was detected. Amplification of tar was succhesfull.

Preparation of chemocompetent cells, LB-media and agarplates. Transformation of BioBrick promoters

Experiment:
Chemocompetent cells and media were prepared like described here. For transformation of BioBrick promoters see Labbook group #2.

Preparation of DH10B with promoter constructs

Experiment:
Over-night culture of DH10B cells. DNA was prepped using peqGOLD MiniPrep Kit (Peqlab).

@@ Line 41: / Line 41: @@
 <b>Amplifcation of the tar gene</b><br>
 <ul>
-<li>Experiment: <br>PCR was performed with isolated genomic DNA from DH10B, forward and reverse primers of <i>tar</i> and using <i>Pfu</i> polymerase. Clean up with PeqGOLD Gelextraction Kit (Peqlab). A band at about 1.6 kb was detected. Amplification of <i>tar</i> was succhesfull.
+<li>Experiment: <br>PCR was performed with isolated genomic DNA from DH10B, forward and reverse primers of <i>tar</i> and using <i>Pfu</i> polymerase. Clean up with peqGOLD Gelextraction Kit (Peqlab). A band at about 1.6 kb was detected. Amplification of <i>tar</i> was succhesfull.
 </li>
 </ul>
@@ Line 65: / Line 65: @@
 <b>Preparation of DH10B with promoter constructs</b><br>
 <ul>
-<li>Experiment: <br>Over-night culture of DH10B cells. DNA was prepped using PeqGOLD MiniPrep Kit (Peqlab).
+<li>Experiment: <br>Over-night culture of DH10B cells. DNA was prepped using peqGOLD MiniPrep Kit (Peqlab).
 </li>
 </ul>