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V05_22
Preparation of plates, solutions
- Experiment:
Preparation of 0.1 M IPTG solution. Preparation of LB-Amp-Xgal/IPTG agar plates.
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V05_23
Amplifcation of the tar gene
- Experiment:
PCR was performed with isolated genomic DNA from DH10B, forward and reverse primers of tar and using Pfu polymerase. Clean up with peqGOLD Gel Extraction Kit (Peqlab). A band at about 1.6 kb was detected. Amplification of tar was succhesfull.
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V05_25
Preparation of chemocompetent cells, LB-media and agarplates. Transformation of BioBrick promoters
- Experiment:
Chemocompetent cells and media were prepared like described here. For transformation of BioBrick promoters see Labbook group #2.
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V05_27
Preparation of DH10B with promoter constructs
- Experiment:
Over-night culture of DH10B cells. DNA was prepped using peqGOLD Miniprep Kit (Peqlab).
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