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Team:Groningen/Notebook/Wetwork 12September2012
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| + | Tom/Renske/Elbrich | ||
Test GFP: reaction to rotten meat | Test GFP: reaction to rotten meat | ||
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microscope results: no GFP, only autofluorescence. | microscope results: no GFP, only autofluorescence. | ||
| + | Renske | ||
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| + | put promoters of LMU Munchen in dh5a | ||
| + | |||
| + | transformation of ~1 uL at 15:00 | ||
| + | mistake! forgot to add LB medium!! --> Plate out anyway (amp plates) + O/N 5 mL culture of the rest.. | ||
| + | |||
| + | |||
| + | O/N culture of: | ||
| + | *rfp-psac-cm in dh5a: 2x from 9/09 (our competent cells) | ||
| + | *rfp-psac-cm in dh5a: 5x from 10/09 (new competent cells) | ||
| + | |||
| + | at 15:00, 37 degrees C. | ||
<html> | <html> | ||
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
Revision as of 13:11, 21 September 2012
Tom/Renske/Elbrich
Test GFP: reaction to rotten meat
meat: 5 gram aliquot from freezer, 3 days at 20 degrees Celsius
tested: Bacillus subtilis sp. 168 _ SboA_GFP in psac-cm (subjected to meat, and without meat) and WT (without meat)
11:00 inoculate O/N culture in SMM in normal 37 degrees stove.
- OD SboA = 0.302
- OD WT = 0.317
12:30 SboA-GFP to micro-array test setup, start OD = 0.1 (see notebook Wetwork 02/09 and 27/06). Also put SboA-GFP and WT in normal 37 degrees stove (start OD = 0.1).
14:30 harvest cells
- OD WT = 1.168
- OD SboA(no meat) = 1.023
- OD SboA(meat) = 1.153
put cells on microscope slide for fluorescence microscopy:
- agarose (1% in SMM) 500 uL on slide
- spread with glass plate
- spot 1 uL of sample on slide (duplo)
microscope results: no GFP, only autofluorescence.
Renske
put promoters of LMU Munchen in dh5a
transformation of ~1 uL at 15:00 mistake! forgot to add LB medium!! --> Plate out anyway (amp plates) + O/N 5 mL culture of the rest..
O/N culture of:
- rfp-psac-cm in dh5a: 2x from 9/09 (our competent cells)
- rfp-psac-cm in dh5a: 5x from 10/09 (new competent cells)
at 15:00, 37 degrees C. Back to notebook
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