Team:Groningen/Construct

From 2012.igem.org

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== Construct ==
 
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The standard construct is a combination of biobricks.
 
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*Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
 
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* BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010.  Other options: BBa_J37019;BBa_K116639; BBaK116609.
 
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* BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.
 
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[[File:Groningen2012_JP_20120618_pRe-CII-Lycopene_Construct_A.png|680px|center]]
 
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* Or, using BBa_K274002 (Reporter) - Violet pigment producer
 
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[[File:Groningen2012_JP_20120618_pRe-CII-Violacein_Construct_A.png|680px|center]]
 

Revision as of 21:27, 17 September 2012




Construct

sticker


germination


volatiles


identification


pigment


construct


death



The standard construct is a combination of biobricks.

  1. Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
  2. BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
  3. BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.










  4. Or, using BBa_K274002 (Reporter) - Violet pigment producer.


Our sponsors:

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