Team:Groningen/Construct

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Revision as of 21:25, 17 September 2012




Construct

sticker


germination


volatiles


identification


pigment


construct


death



The standard construct is a combination of biobricks.

  1. Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
  2. BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
  3. BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.








  4. Or, using BBa_K274002 (Reporter) - Violet pigment producer.


Our sponsors:


Construct

The standard construct is a combination of biobricks.

  • Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
  • BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
  • BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.
Groningen2012 JP 20120618 pRe-CII-Lycopene Construct A.png
  • Or, using BBa_K274002 (Reporter) - Violet pigment producer
Groningen2012 JP 20120618 pRe-CII-Violacein Construct A.png
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