"
Team:Groningen/Notebook/Wetwork 7July2012
From 2012.igem.org
| Line 1: | Line 1: | ||
{{HeaderGroningen2012}} | {{HeaderGroningen2012}} | ||
| - | + | <div style="margin-left: 100px; margin-right:100px; color:white; font-size:12pt;"> | |
Emeraldo | Emeraldo | ||
| Line 14: | Line 14: | ||
Purified cDNA as [[Team:Groningen/Notebook/Wetwork_5July2012|previously]]. Concentrations far too low (22.1 ng/uL). RNA degraded? Wrong handling? --> Ask Arjan to repeat work on Monday. | Purified cDNA as [[Team:Groningen/Notebook/Wetwork_5July2012|previously]]. Concentrations far too low (22.1 ng/uL). RNA degraded? Wrong handling? --> Ask Arjan to repeat work on Monday. | ||
| + | |||
| + | <html> | ||
| + | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
| + | </html> | ||
| + | |||
| + | {{Template:SponsorsGroningen2012}} | ||
Revision as of 17:03, 16 September 2012
Emeraldo
1.) pSac-CM isolated yield 200ng/ul
2.) Gel run of gradient PCR. All primers can amplify their fragments in all temperatures: 150alsTF+Rev amplifies 150bp fragment, 250alsTF+Rev amplifies 250bp fragment, 300alsTF+Rev amplifies 300bp fragment, and 500alsTF+Rev amplifies 500bp fragment. Optimal annealing temperature: 58 degree celcius until 60 degree celcius, for all primer sets.
3.) B.subtilis transformation yesterday was a success for pSac-CM. Both methods show transformants. For BBa_I742123: both methods show no transformant. This is not expected. Ask team Edinburgh?
Renske
Purified cDNA as previously. Concentrations far too low (22.1 ng/uL). RNA degraded? Wrong handling? --> Ask Arjan to repeat work on Monday.
Our sponsors:
