Team:Groningen/parts fail

From 2012.igem.org

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<p>Failed BioBricks
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BBa_K090403
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<p>Failed BioBricks<br>
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'''Name'''  Gram-positive Shuttle Vector for Chromosomal Integration
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<b>BBa_K090403</b><br>
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<b>Name</b> Gram-positive Shuttle Vector for Chromosomal Integration<br>
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'''Intended Purpose'''  Plasmid backbone for cloning in ''Bacillus subtilis''
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<b>Intended Purpose</b> Plasmid backbone for cloning in <i>Bacillus subtilis</i><br>
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<b>Testing Protocol<b> Restriction analysis (single cut)<br>
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'''Testing Protocol''' Restriction analysis (single cut)
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<b>Results<b> The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is shorter than the expected size.<br>
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'''Results''' The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is shorter than the expected size.
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<b>BBa_I742123</b><br>
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<b>Name</b> multi-host vector pTG262 converted to BioBrick vector<br>
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BBa_I742123
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<b>Intended Purpose</b> plasmid backbone for cloning in <i>Bacilus subtilis</i><br>
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<b>Testing Protocol</b> restriction analysis (single cut) and restriction analysis (dual cut)<br>
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'''Name''' multi-host vector pTG262 converted to BioBrick vector
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<b>Results</b> the restriction enzymes cut at illegal site(s)<br>
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'''Intended Purpose''' plasmid backbone for cloning in ''Bacilus subtilis''
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'''Testing Protocol''' restriction analysis (single cut) and restriction analysis (dual cut)
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'''Results''' the restriction enzymes cut at illegal site(s)
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{{Template:SponsorsGroningen2012}}
{{Template:SponsorsGroningen2012}}

Revision as of 00:41, 16 September 2012




Failed BioBricks

BBa_K090403
Name Gram-positive Shuttle Vector for Chromosomal Integration
Intended Purpose Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol Restriction analysis (single cut)
Results The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is shorter than the expected size.

BBa_I742123
Name multi-host vector pTG262 converted to BioBrick vector
Intended Purpose plasmid backbone for cloning in Bacilus subtilis
Testing Protocol restriction analysis (single cut) and restriction analysis (dual cut)
Results the restriction enzymes cut at illegal site(s)

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