Team:Goettingen/week8-2


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#2 Speed Improvement - 8th week

Chemical transformation of the BioBrick plasmid pSB1C3 into E. coli (DH10B)

Experiment:
In order to gain further plasmid material of the new vector, pSB1C3 was transformed into E. coli (DH10B) as described in the protocol.
Observations & Results:
The transformation was not successful. No colonies could be observed at the plates. Since this vector has a chloramphenicol resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotic concentration in the agar was simply to high.

V06_19_1 Repetition of the chemical transformation of the BioBrick plasmid pSB1C3 into E. coli (DH10B)

Experiment:
pSB1C3 was transformed into E. coli (DH10B) as described in the protocol.
Observations & Results:
The transformation was successful.Due to the possession of a gene encoding for RFP the developed colonies featured a red color. On the negative control no colonies were observed.

V06_19_2 Repetition of the chemical transformation of the araC-promoter into E. coli (DH10B)

Experiment:
Since the last time the fact that the araC-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only derogation from the standard transformation protocol.
Observations & Results:
The transformation was again not successful. Only the positive control showed any growth.

Preparation of over night cultures