Team:TMU-Tokyo/Parts

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Parts

<groupparts>iGEM012 TMU-Tokyo</groupparts>

■BBa_K749025

pfrm-frmR-E0040-B0015-J23100-B0034-fdh-B0015

■BBa_K749015

J23100-B0034-fdh4A-fdh4B-B0015

■BBa_K749021

pfrm-frmR-E0040-B0015

Formaldehyde monitoring and dehydrogenate device. The upstream is sensor for formaldehyde (Device1) and the downstream consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida (Device2).
BBa_K749025, by itself, enables to observe the degree of formaldehyde decomposition with the naked eye.

Formate dehydrogenase device. BBa_K749015 produces formate dehydrogenase 4 from Methylobacterium extorquens consecutively (Device3).
Complete formaldehyde detoxification is realized by only BBa_K749025 and BBa_K749015.

Upstream of BBa_K749025, formaldehyde sensor. we use BBa_K749021 in assay for frmR inhibitory.