Team:OUC-China
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- | + | <div id="menu-wrapper"> | |
- | + | <div id="logo"> | |
- | + | </div> | |
- | + | <ul class="menu"> | |
- | + | <li> <a href="#">Index</a> </li> | |
- | + | <li> <a href="https://2012.igem.org/Team:OUC-China/Project/Overview">Project</a> </li> | |
- | < | + | <li> <a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview">Modeling</a> </li> |
- | < | + | <li> <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Overview">Human Practice</a> </li> |
+ | <li> <a href="https://2012.igem.org/Team:OUC-China/Team/members">Team</a> </li> | ||
+ | <li> <a href="https://2012.igem.org/Team:OUC-China/Parts">Parts</a> </li> | ||
+ | <li> <a href="https://2012.igem.org/Team:OUC-China/Safety">Safety</a> </li> | ||
+ | <li> <a href="https://2012.igem.org/Team:OUC-China/labnote">Note</a> </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div id="submenu-wrapper"> | ||
+ | <ul class="submenu"> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/a/af/Projectoverview.jpg" /> | ||
+ | Home | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China#hmo"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/4/49/Humanpracticeoverview.jpg" /> | ||
+ | HumanPractice index | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Judgingform"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/6/6e/Juding-form.jpg" /> | ||
+ | JudgingForm | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/contactus"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/1/1e/Contact-us.jpg" /> | ||
+ | Contact Us | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | </ul> | ||
+ | <ul class="submenu"> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Project/Overview"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/5/59/Ouc-nav-project-overview.jpg" /> | ||
+ | Project Overview | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Project/Sensor/AbstractandBackground"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/1/15/Ouc-nav-project-secsor.jpg" /> | ||
+ | Sensor | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/3/34/Ouc-nav-device.jpg" /> | ||
+ | Decision-making Device | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/5/57/Ouc-nav-project-gvp.jpg" /> | ||
+ | Gas vesicle | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <ul class="submenu"> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Modeling/ODEModel"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/f/f6/Ouc-nav-modeling-ode.jpg" /> | ||
+ | ODEModel | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Modeling/ParameterSensitivityAnalysis"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/c/cf/Ouc-nav-modeling-sensertitive.jpg" /> | ||
+ | Parameter Sensitivity Analysis | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Modeling/ParameterSweep"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/0/07/Ouc-nav-modeling-sweep.jpg" /> | ||
+ | Parameter Sweep | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Modeling/NoiseAnalysis"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/0/04/Ouc-nav-modeling-noise.jpg" /> | ||
+ | Noise Analysis | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <ul class="submenu"> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Overview"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/6/61/Ouc-nav-humanoverview.jpg" /> | ||
+ | HumanPractice Overview | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/c/cc/Ouc-nav-Communication.jpg" /> | ||
+ | Meeting and Academic Communication | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/8/8d/Ouc-nav-humcamp.jpg" /> | ||
+ | Camps, Class and Lectures | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/b/b7/Ouc-nav-hmspcial.jpg" /> | ||
+ | Special HP | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <ul class="submenu"> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Team/members"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/6/6d/Ouc-nav-team.jpg" /> | ||
+ | Team Members | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Team/instructor"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/3/35/Ouc-nav-instructor.jpg" /> | ||
+ | Instructors | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Team/AcknowledgementandCooperation"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/a/a6/Ouc-nav-co.jpg" /> | ||
+ | Acknowledgement&Cooperation | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Team/Lab"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/a/a7/Ouc-nav-lab.jpg" /> | ||
+ | Lab | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <ul class="submenu"> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Parts"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/f/f9/Ouc-nav-parts.jpg" /> | ||
+ | Parts | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <ul class="submenu"> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Safety"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/5/5d/Ouc-nav-safety.jpg" /> | ||
+ | Safety | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <ul class="submenu"> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/labnote"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/8/8b/Ouc-nav-labnote.jpg" /> | ||
+ | Labnote | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://skydrive.live.com/redir?resid=6A13E80E2A176A23!249&authkey=!AGsNOi9excznIt8"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/4/4e/Ouc-nav-modelingnote.jpg" /> | ||
+ | Modeling Note | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/protocol"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/7/7d/Ouc-pro.png" /> | ||
+ | Protocols | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div class="container"> | ||
+ | <div id="da-slider" class="da-slider"> | ||
+ | <div class="da-slide"> | ||
+ | <h2 >Overview</h2> | ||
+ | <p>Red tide detector: | ||
+ | Facilitate small RNA as information processing media; | ||
<br/> | <br/> | ||
- | + | and gas vesicle as a novel output actuator... | |
- | + | ||
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- | and | + | |
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- | + | ||
<br/> | <br/> | ||
- | < | + | Response to the overloading phosphate or nitrate when they exceeds warning limit...<br/> |
- | <p> | + | </p> |
+ | <a href="https://2012.igem.org/Team:OUC-China/Project/Overview" class="da-link">Read more</a> | ||
+ | <div class="da-img"><img src="https://static.igem.org/mediawiki/2012/1/19/Ouc-final.png" alt="overview" /></div> | ||
+ | </div> | ||
+ | <div class="da-slide"> | ||
+ | <h2>Sensor</h2> | ||
+ | <p>The sensors for next-generation detector of red tide!</p> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Project/Sensor/AbstractandBackground" class="da-link">Read more</a> | ||
+ | <div class="da-img"><img src="https://static.igem.org/mediawiki/2012/3/32/Home-sensor.png" alt="sensor" /></div> | ||
+ | </div> | ||
+ | <div class="da-slide"> | ||
+ | <h2>Model-Driven Design</h2> | ||
+ | <p> | ||
+ | Get specific directions for ternary system design from simulation... | ||
+ | </p> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview" class="da-link">Read more</a> | ||
+ | <div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/26/Home-modeling.png" alt="Home-modeling" /></div> | ||
+ | </div> | ||
+ | <div class="da-slide"> | ||
+ | <h2>Making decision through sRNA</h2> | ||
+ | <p>The sophisticated 'CPU' of our red tide detector...<br/> | ||
+ | Engineering CPU for your own "synthia"!</p> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract" class="da-link">Read more</a> | ||
+ | <div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/2c/Home-decision-making-device.png" alt="Home-decision-making-device" /></div> | ||
+ | </div> | ||
- | + | <div class="da-slide"> | |
- | + | <h2>Floating Device</h2> | |
- | + | <p>The output actuator of our red tide detector...<br/> | |
- | a | + | Gas vesicle as our floating device</p> |
- | + | <a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground" class="da-link">Read more</a> | |
- | + | <div class="da-img"><img src="https://static.igem.org/mediawiki/2012/a/a2/Ouc-home-floating-device.png" /></div> | |
+ | </div> | ||
+ | <nav class="da-arrows"> | ||
+ | <span class="da-arrows-prev"></span> | ||
+ | <span class="da-arrows-next"></span> | ||
+ | </nav> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div id="project_intro_box"> | ||
+ | <div class="project_intro"> | ||
+ | <h2>Oceanfloat and Oceanfeel<br/><span>——a floating biosensor</span></h2> | ||
+ | </div> | ||
+ | </div> | ||
+ | <section id="project_step"> | ||
+ | <div class="moduletable"> | ||
+ | <h3>Our Project</h3> | ||
+ | <div> | ||
+ | <ul id="tab"> | ||
+ | <li class="on" id="tab_1" onclick="switchTab(1)"><img src="https://static.igem.org/mediawiki/2012/8/8f/Ouc-home-step1.png" /><span>Part1</span></li> | ||
+ | <li id="tab_2" onclick="switchTab(2)"><img src="https://static.igem.org/mediawiki/2012/d/de/Ouc-home-step3.png" /><span>Part2</span></li> | ||
+ | <li id="tab_3" onclick="switchTab(3)"><img src="https://static.igem.org/mediawiki/2012/a/a4/Ouc-home-steplast.png" /><span>Part3</span></li> | ||
+ | </ul> | ||
+ | <div style="clear: both;"> </div> | ||
+ | <div class="tuxing"><img src="https://static.igem.org/mediawiki/2012/e/e2/Ouc-hometab_arrow.png" /></div> | ||
+ | <ul id="tab_con"> | ||
+ | <li id="tab_con_1"> | ||
+ | <div class="tup"><img src="https://static.igem.org/mediawiki/2012/3/3a/Ouc-home-step1-2.png" /></div> | ||
+ | <div class="nr"> | ||
+ | <h1>Sensor</h1> | ||
+ | <p>Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.</p> | ||
+ | <p>much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.</p> | ||
+ | <p><a href="https://2012.igem.org/Team:OUC-China/Project/Sensor/AbstractandBackground"><strong>learn more</strong></a> | ||
+ | </div> | ||
+ | <div style="clear: both;"> </div> | ||
+ | </li> | ||
+ | <li id="tab_con_2"> | ||
+ | <div class="tup"><img src="https://static.igem.org/mediawiki/2012/a/a1/Ouc-home-step3-02.png" /></div> | ||
+ | <div class="nr"> | ||
+ | <h1>Decision-making Device</h1> | ||
+ | <p>Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.</p> | ||
+ | <p>Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).</p> | ||
+ | <p><a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract"><strong>learn more</strong></a> | ||
+ | </div> | ||
+ | <div style="clear: both;"> </div> | ||
+ | </li> | ||
+ | <li id="tab_con_3"> | ||
+ | <div class="tup"><img src="https://static.igem.org/mediawiki/2012/f/fa/Ouc-step3-last.png" /></div> | ||
+ | <div class="nr"> | ||
+ | <h1>Floating Device</h1> | ||
+ | <p>In our project, by transforming the buoyancy device BBa_K737010(designed by ourselves), the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.</p> | ||
+ | <p><a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground"><strong>learn more</strong></a> | ||
+ | </div> | ||
+ | <div style="clear: both;"> </div> | ||
+ | </li> | ||
+ | </ul></div> | ||
+ | </div> | ||
+ | </section> | ||
+ | <div class="hmoverview" id="hmo"></div> | ||
+ | <div class="hmguide"> | ||
+ | <h3>Human practice</h3> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/d7/Ouc-homehp01.jpg" alt="Meeting and Academic Communication" title="Meeting and Academic Communication" /></a> | ||
+ | <h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank">Meeting and Academic Communication</a></h4> | ||
+ | <p>As a new born iGEM team,questions below puzzled us for a very long time. However,Peking Univerisity iGEM team helped us a lot by giving us dire……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank">[Learn more]</a></span></p> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank"><img src="https://static.igem.org/mediawiki/2012/a/a5/Ouc-homehp02.jpg" alt="Camps, Class and Lectures" title="Camps, Class and Lectures" /></a> | ||
+ | <h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank">Camps, Class and Lectures</a></h4> | ||
+ | <p>Last year, we held 1st summer camp of life science and technology. Through this camp, most of the participants……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank">[Learn more]</a></span></p> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank"><img src="https://static.igem.org/mediawiki/2012/4/40/Ouc-homehp03.jpg" alt="Special OUC-China HP" title="Special OUC-China HP" /></a> | ||
+ | <h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank">Special OUC-China HP</a></h4> | ||
+ | <p>Because our project of this focuses on the ratio of N and P and cyanobacteria in the sea water, we consulted professors who study on ecology……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank">[Learn more]</a></span></p> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
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+ | <a href="https://2012.igem.org/Team:OUC-China/contactus"><img src="https://static.igem.org/mediawiki/2012/5/5e/OUC-footer-contact.jpg" alt="Contact Us" border="0"></a> | ||
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Latest revision as of 13:25, 26 October 2012
Oceanfloat and Oceanfeel
——a floating biosensor
Our Project
Part1
Part2
Part3
![](https://static.igem.org/mediawiki/2012/e/e2/Ouc-hometab_arrow.png)
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Sensor
Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.
much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.
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Decision-making Device
Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.
Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).
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Floating Device
In our project, by transforming the buoyancy device BBa_K737010(designed by ourselves), the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.
Human practice
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Meeting and Academic Communication
As a new born iGEM team,questions below puzzled us for a very long time. However,Peking Univerisity iGEM team helped us a lot by giving us dire……[Learn more]
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Camps, Class and Lectures
Last year, we held 1st summer camp of life science and technology. Through this camp, most of the participants……[Learn more]
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Special OUC-China HP
Because our project of this focuses on the ratio of N and P and cyanobacteria in the sea water, we consulted professors who study on ecology……[Learn more]