Team:OUC-China

From 2012.igem.org

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<div class="index_header_wrp">
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<div id="menu-wrapper">
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<div id="logo">
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        <a href="" class="login_logo"></a>
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</div>
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<ul class="menu">
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</div>
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<li> <a href="#">Index</a> </li>
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<div class="index_bg">
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<li> <a href="https://2012.igem.org/Team:OUC-China/Project/Overview">Project</a> </li>
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<ul id="nav">
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<li> <a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview">Modeling</a> </li>
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<li><a href="">Home</a></li>
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<li> <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Overview">Human Practice</a> </li>
-
<li><a href="">Team</a></li>
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<li> <a href="https://2012.igem.org/Team:OUC-China/Team/members">Team</a> </li>
-
<li><a href="">Project</a></li>
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<li> <a href="https://2012.igem.org/Team:OUC-China/Parts">Parts</a> </li>
-
<li><a href="">Modeling</a></li>
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<li> <a href="https://2012.igem.org/Team:OUC-China/Safety">Safety</a> </li>
-
<li><a href="">Notebook</a></li>
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<li> <a href="https://2012.igem.org/Team:OUC-China/labnote">Note</a> </li>
-
<li><a href="">Safety</a></li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China">
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                            <img src="https://static.igem.org/mediawiki/2012/e/e2/OUC-China-KangLi.jpg" alt="Kang Li"/>
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<img src="https://static.igem.org/mediawiki/2012/a/af/Projectoverview.jpg" />
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                            <div class="ei-title">
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Home
-
                                <h2>Captain </h2>
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</a>
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                                <h3>Kang Li</h3>
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</li>
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                            </div>
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<li>
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                        </li>
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<a href="https://2012.igem.org/Team:OUC-China#hmo">
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                        <li>
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<img src="https://static.igem.org/mediawiki/2012/4/49/Humanpracticeoverview.jpg" />
-
                            <img src="https://static.igem.org/mediawiki/2012/e/e2/OUC-China-KangLi.jpg" alt="image01" />
+
HumanPractice index
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                            <div class="ei-title">
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</a>
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                                <h2>Creative</h2>
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</li>
-
                                <h3>Geek</h3>
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<li>
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                            </div>
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<a href="https://2012.igem.org/Team:OUC-China/Judgingform">
-
                        </li>
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<img src="https://static.igem.org/mediawiki/2012/6/6e/Juding-form.jpg" />
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                        <li>
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JudgingForm
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                            <img src="https://static.igem.org/mediawiki/2012/e/e2/OUC-China-KangLi.jpg" alt="image02" />
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</a>
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                            <div class="ei-title">
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</li>
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                                <h2>Friendly</h2>
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<li>
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                                <h3>Devil</h3>
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<a href="https://2012.igem.org/Team:OUC-China/contactus">
-
                            </div>
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<img src="https://static.igem.org/mediawiki/2012/1/1e/Contact-us.jpg" />
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                        </li>
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Contact Us
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                        <li>
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</a>
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                            <img src="https://static.igem.org/mediawiki/2012/e/e2/OUC-China-KangLi.jpg" alt="image03"/>
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                            <div class="ei-title">
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</li>
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                                <h2>Tranquilent</h2>
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</ul>
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                                <h3>Compatriot</h3>
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<ul class="submenu">
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                            </div>
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<li>
-
                        </li>
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<a href="https://2012.igem.org/Team:OUC-China/Project/Overview">
-
                        <li>
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<img src="https://static.igem.org/mediawiki/2012/5/59/Ouc-nav-project-overview.jpg" />
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                            <img src="https://static.igem.org/mediawiki/2012/e/e2/OUC-China-KangLi.jpg" alt="image04"/>
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Project Overview
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                            <div class="ei-title">
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</a>
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                                <h2>Insecure</h2>
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</li>
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                                <h3>Hussler</h3>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/Project/Sensor/AbstractandBackground">
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<img src="https://static.igem.org/mediawiki/2012/1/15/Ouc-nav-project-secsor.jpg" />
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Sensor
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                            <img src="https://static.igem.org/mediawiki/2012/e/e2/OUC-China-KangLi.jpg" alt="image05"/>
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</a>
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                            <div class="ei-title">
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                                <h2>Loving</h2>
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<li>
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                                <h3>Rebel</h3>
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<a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract">
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                            </div>
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<img src="https://static.igem.org/mediawiki/2012/3/34/Ouc-nav-device.jpg" />
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Decision-making Device
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                        <li>
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</a>
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                            <img src="https://static.igem.org/mediawiki/2012/e/e2/OUC-China-KangLi.jpg" alt="image07"/>
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</li>
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                            <div class="ei-title">
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<li>
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                                <h2>Photography by</h2>
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<a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground">
-
                                <h3>Peng Yong</a></h3>
+
<img src="https://static.igem.org/mediawiki/2012/5/57/Ouc-nav-project-gvp.jpg" />
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                            </div>
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Gas vesicle
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                        </li>
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</a>
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</li>
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                    <ul class="ei-slider-thumbs">
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</ul>
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                        <li class="ei-slider-element">Current</li>
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<ul class="submenu">
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<li><a href="#">Slide 6</a><img src="images/thumbs/6.jpg" alt="thumb06" /></li>
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<li>
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                        <li><a href="#">Slide 1</a><img src="images/thumbs/1.jpg" alt="thumb01" /></li>
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<a href="https://2012.igem.org/Team:OUC-China/Modeling/ODEModel">
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                        <li><a href="#">Slide 2</a><img src="images/thumbs/2.jpg" alt="thumb02" /></li>
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<img src="https://static.igem.org/mediawiki/2012/f/f6/Ouc-nav-modeling-ode.jpg" />
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                        <li><a href="#">Slide 3</a><img src="images/thumbs/3.jpg" alt="thumb03" /></li>
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ODEModel
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                        <li><a href="#">Slide 4</a><img src="images/thumbs/4.jpg" alt="thumb04" /></li>
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</a>
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                        <li><a href="#">Slide 5</a><img src="images/thumbs/5.jpg" alt="thumb05" /></li>
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                        <li><a href="#">Slide 7</a><img src="images/thumbs/7.jpg" alt="thumb07" /></li>
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<a href="https://2012.igem.org/Team:OUC-China/Modeling/ParameterSensitivityAnalysis">
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<img src="https://static.igem.org/mediawiki/2012/c/cf/Ouc-nav-modeling-sensertitive.jpg" />
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Parameter Sensitivity Analysis
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<div class="text">
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</a>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/Modeling/ParameterSweep">
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<img src="https://static.igem.org/mediawiki/2012/0/07/Ouc-nav-modeling-sweep.jpg" />
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Parameter Sweep
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/Modeling/NoiseAnalysis">
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<img src="https://static.igem.org/mediawiki/2012/0/04/Ouc-nav-modeling-noise.jpg" />
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Noise Analysis
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</a>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Overview">
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<img src="https://static.igem.org/mediawiki/2012/6/61/Ouc-nav-humanoverview.jpg" />
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HumanPractice Overview
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication">
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<img src="https://static.igem.org/mediawiki/2012/c/cc/Ouc-nav-Communication.jpg" />
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Meeting and Academic Communication
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps">
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<img src="https://static.igem.org/mediawiki/2012/8/8d/Ouc-nav-humcamp.jpg" />
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Camps, Class and Lectures
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</a>
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</li>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM">
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<img src="https://static.igem.org/mediawiki/2012/b/b7/Ouc-nav-hmspcial.jpg" />
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Special HP
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Team Members
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</a>
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<a href="https://2012.igem.org/Team:OUC-China/Team/instructor">
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<img src="https://static.igem.org/mediawiki/2012/3/35/Ouc-nav-instructor.jpg" />
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Instructors
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</a>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/Team/AcknowledgementandCooperation">
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<img src="https://static.igem.org/mediawiki/2012/a/a6/Ouc-nav-co.jpg" />
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Acknowledgement&Cooperation
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/Team/Lab">
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<img src="https://static.igem.org/mediawiki/2012/a/a7/Ouc-nav-lab.jpg" />
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Lab
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<ul class="submenu">
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Parts
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</a>
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Safety
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<a href="https://2012.igem.org/Team:OUC-China/labnote">
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Labnote
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</a>
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<a href="https://skydrive.live.com/redir?resid=6A13E80E2A176A23!249&authkey=!AGsNOi9excznIt8">
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<img src="https://static.igem.org/mediawiki/2012/4/4e/Ouc-nav-modelingnote.jpg" />
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Modeling Note
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</a>
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<a href="https://2012.igem.org/Team:OUC-China/protocol">
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Protocols
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</a>
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</li>
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</ul>
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</div>
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<div class="container">
 +
<div id="da-slider" class="da-slider">
 +
<div class="da-slide">
 +
<h2 >Overview</h2>
 +
<p>Red tide detector:
 +
Facilitate small RNA as information processing media;
<br/>
<br/>
-
<h1>Description of our project</h1>
+
and gas vesicle as a novel output actuator...
-
<h2>Introduction: motivation and background</h2>
+
<br/>
-
<p>This year, our projects focus on biological methods for warning and countermeasure against water bloom and red tide. As we know, this ecological disaster, due to
+
Response to the overloading phosphate or nitrate when they exceeds warning limit...<br/>
 +
</p>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/Overview" class="da-link">Read more</a>
 +
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/1/19/Ouc-final.png" alt="overview" /></div>
 +
</div>
 +
<div class="da-slide">
 +
<h2>Sensor</h2>
 +
<p>The sensors for next-generation detector of red tide!</p>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/Sensor/AbstractandBackground" class="da-link">Read more</a>
 +
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/3/32/Home-sensor.png" alt="sensor" /></div>
 +
</div>
 +
<div class="da-slide">
 +
<h2>Model-Driven Design</h2>
 +
<p>
 +
Get specific directions for ternary system design from simulation...
 +
</p>
 +
<a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview" class="da-link">Read more</a>
 +
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/26/Home-modeling.png" alt="Home-modeling" /></div>
 +
</div>
 +
<div class="da-slide">
 +
<h2>Making decision through sRNA</h2>
 +
<p>The sophisticated  'CPU' of our red tide detector...<br/>
 +
Engineering CPU for your own "synthia"!</p>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract" class="da-link">Read more</a>
 +
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/2c/Home-decision-making-device.png" alt="Home-decision-making-device" /></div>
 +
</div>
-
nutrient overload and followed by oxygen deprivation, tortured many countries and caused gigantic economic loss and ecological disruption. We propose that to prevent
+
<div class="da-slide">
-
 
+
<h2>Floating Device</h2>
-
it happen, a precise sensor for alarming the arrival of water bloom and an effective actuator to solve it are needed. So our projects can be summarized as two parts:
+
<p>The output actuator of our red tide detector...<br/>
-
The first goal is to develop a synthetic system which can sense the outer signal and have a precise decision-making about to what degree will there be a water bloom.
+
Gas vesicle as our floating device</p>
-
 
+
<a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground" class="da-link">Read more</a>
-
We will facilitate a fine-tuned ratio sensor sensing the ratio of nitrogen and phosphorus to achieve this.<br/>
+
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/a/a2/Ouc-home-floating-device.png" /></div>
-
The second goal is to construct an actuator with ‘portable’ gas vesicles which enable our engineered microbes to float on the water surface.<br/>
+
</div>
-
The third goal is to provide our actuator with several functions making it a versatile cleaner to contain the water bloom.</p>
+
<nav class="da-arrows">
-
<h2>What trigger water bloom?</h2>
+
<span class="da-arrows-prev"></span>
-
<p>The well-recognized culprit of water bloom is overload of nutrient (eutrophication).Take the Baltic Sea as example, dumping from sewage-treatment plants, farming
+
<span class="da-arrows-next"></span>
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+
</nav>
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and industry has poured about 20 million tons of nitrogen and 2 million tons of phosphorus into the Baltic over the past 50 years, which give rise to long-lasting
+
</div>
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+
        </div>
-
algal blooms. The net effect is an excess of phosphorous, which fuels nitrogen-fixing cyanobacteria and triggers algal blooms as the essential condition. Another
+
<div id="project_intro_box">
-
 
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<div class="project_intro">
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crucial factor is an excess of nitrogen which can amplify the effect and enhance overgrowth of the cyanobacteria and alga. And many research indicate that many a
+
<h2>Oceanfloat and Oceanfeel<br/><span>——a floating biosensor</span></h2>
-
 
+
</div>
-
particular ratio of this two element, relatively stable, coincide with the water bloom. It suggests that a ratio sensor to alarm for water bloom is reliable.</p>
+
</div>
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+
<section id="project_step">
-
<h2>What is a ratio sensor?</h2>
+
<div class="moduletable">
-
<p>As we have mentioned, a ratio sensor could be an indicator for the arrival of water bloom. But only when we make a fine-tuned ratio sensor which can sense the two
+
<h3>Our Project</h3>
-
 
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<div>
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different signals(nitrogen&phosphorus) precisely, account the ratio accurately and perform a remarkable reporting signal, can we assure that it is a fine-tuned and
+
<ul id="tab">
-
 
+
<li class="on" id="tab_1" onclick="switchTab(1)"><img src="https://static.igem.org/mediawiki/2012/8/8f/Ouc-home-step1.png" /><span>Part1</span></li>
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reliable alarm.<br/>
+
<li id="tab_2" onclick="switchTab(2)"><img src="https://static.igem.org/mediawiki/2012/d/de/Ouc-home-step3.png" /><span>Part2</span></li>
-
a) We will facilitate phoB promoter and NtrB promoter for sensing phosphorus and nitrogen respectively.<br/>
+
<li id="tab_3" onclick="switchTab(3)"><img src="https://static.igem.org/mediawiki/2012/a/a4/Ouc-home-steplast.png" /><span>Part3</span></li>
-
b) Ratio accounting is implemented with an artificial system of RNA –mediated regulation.<br/>
+
</ul>
-
c) We choose GFP as the reporter to show our result.<br/>
+
<div style="clear: both;">&nbsp;</div>
-
<h2>What is gas vesicle?</h2>
+
<div class="tuxing"><img src="https://static.igem.org/mediawiki/2012/e/e2/Ouc-hometab_arrow.png" /></div>
-
<p>To facilitate our E.coli to float on the water surface, we should equip our engineered E.coli with portable generators of gas vesicles. The gas vesicle is a hollow
+
<ul id="tab_con">
-
 
+
<li id="tab_con_1">
-
structure made of protein. It usually has the form of a cylindrical tube closed by conical end caps. Gas vesicles provide aquatic microbes with buoyancy so that they  
+
<div class="tup"><img src="https://static.igem.org/mediawiki/2012/3/3a/Ouc-home-step1-2.png" /></div>
-
 
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<div class="nr">
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can control their vertical migration. It could have been a great tool to hold our engineered E.coli on the water surface, as the previous teams had shown us. However,
+
<h1>Sensor</h1>
-
 
+
<p>Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.</p>
-
due to the horrible length of its coding genes and the heavy burden it brought about to its host, the practical application remain difficult and uncertain. Our team
+
<p>much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.</p>
-
 
+
<p><a href="https://2012.igem.org/Team:OUC-China/Project/Sensor/AbstractandBackground"><strong>learn more</strong></a>
-
determines to break through the task by fine-tuning the expression pattern of gvpA and gvpC which test the most economical way to provide buoyancy.</p>
+
</div>
-
<h2>How can our actuator curb the water bloom?</h2>
+
<div style="clear: both;">&nbsp;</div>
-
<p>So if the two projects above go well, we will focus on constructing a versatile warrior which can stifle the water bloom in the cradle. We may take advantage of
+
</li>
-
 
+
<li id="tab_con_2">
-
some genetic parts  to absorb and fix the nutrients , release toxins restricted to the toxic cyanobacteria and alga and even facilitate the mechanism of vertical
+
<div class="tup"><img src="https://static.igem.org/mediawiki/2012/a/a1/Ouc-home-step3-02.png" /></div>
-
 
+
<div class="nr">
-
migration thereby bring the nutrients to the deeper area.</p>
+
<h1>Decision-making Device</h1>
 +
<p>Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.</p>
 +
<p>Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).</p>
 +
<p><a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract"><strong>learn more</strong></a>
</div>
</div>
-
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.7.0/jquery.min.js"></script>
+
<div style="clear: both;">&nbsp;</div>
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<script>
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</li>
-
    (function( window, $, undefined ) {
+
<li id="tab_con_3">
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<div class="tup"><img src="https://static.igem.org/mediawiki/2012/f/fa/Ouc-step3-last.png" /></div>
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/*
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<div class="nr">
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* smartresize: debounced resize event for jQuery
+
<h1>Floating Device</h1>
-
*
+
<p>In our project, by transforming the buoyancy device BBa_K737010(designed by ourselves), the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.</p>
-
* latest version and complete README available on Github:
+
<p><a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground"><strong>learn more</strong></a>
-
* https://github.com/louisremi/jquery.smartresize.js
+
</div>
-
*
+
<div style="clear: both;">&nbsp;</div>
-
* Copyright 2011 @louis_remi
+
</li>
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* Licensed under the MIT license.
+
</ul></div>
-
*/
+
</div>
 +
</section>
 +
<div class="hmoverview" id="hmo"></div>
 +
<div class="hmguide">
 +
<h3>Human practice</h3>
 +
<ul>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/d7/Ouc-homehp01.jpg" alt="Meeting and Academic Communication" title="Meeting and Academic Communication" /></a>
 +
<h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank">Meeting and Academic Communication</a></h4>
 +
<p>As a new born iGEM team,questions below puzzled us for a very long time. However,Peking Univerisity iGEM team helped us a lot by giving us dire……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank">[Learn more]</a></span></p>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank"><img src="https://static.igem.org/mediawiki/2012/a/a5/Ouc-homehp02.jpg" alt="Camps, Class and Lectures" title="Camps, Class and Lectures" /></a>
 +
<h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank">Camps, Class and Lectures</a></h4>
 +
<p>Last year, we held 1st summer camp of life science and technology. Through this camp, most of the participants……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank">[Learn more]</a></span></p>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank"><img src="https://static.igem.org/mediawiki/2012/4/40/Ouc-homehp03.jpg" alt="Special OUC-China HP" title="Special OUC-China HP" /></a>
 +
<h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank">Special OUC-China HP</a></h4>
 +
<p>Because our project of this focuses on the ratio of N and P and cyanobacteria in the sea water, we consulted professors who study on ecology……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank">[Learn more]</a></span></p>
 +
</li>
 +
</ul>
 +
</div>
 +
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                <li>
 +
<a href="http://www.renren.com/296396903/profile?ref=opensearch_normal"><img src="https://static.igem.org/mediawiki/2012/d/d2/Ouc-footer-cmlsast.jpg" alt="OUC CMLS AST" border="0"></a> 
 +
                </li>
 +
                <li>
 +
                    <a href="https://2012.igem.org/Team:OUC-China/contactus"><img src="https://static.igem.org/mediawiki/2012/5/5e/OUC-footer-contact.jpg" alt="Contact Us" border="0"></a>
 +
                </li>
 +
                <li style="width:160px;">
 +
<form action="/Special:Search" id="searchform" style=" border-color:#000;">
 +
<input id="searchInput" name="search" type="text" title="Search 2011.igem.org [f]" accesskey="f" value="">
 +
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;
 +
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
 +
</form>
 +
                </li>
 +
            </ul>
 +
        </div>
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-
$.Slideshow.prototype = {
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this.options = $.extend( true, {}, $.Slideshow.defaults, options );
+
$('#da-slider').cslider({
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-
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-
+
-
$.when( this._preloadImages() ).done( function() {
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+
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-
_self.$loading.hide();
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-
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-
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_self.$imgItems.eq( _self.current ).css({
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if( _self.options.autoplay ) {
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var _self = this,
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+
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).promise();
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+
-
},
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-
+
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// save ei-slider's width
+
-
this.elWidth = this.$el.width();
+
-
+
-
var _self = this;
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-
+
-
this.$images.each( function( i ) {
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-
+
-
var $img = $(this);
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+
-
+
-
$img.css({
+
-
width : imgDim.width,
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-
height : imgDim.height,
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-
marginLeft : imgDim.left,
+
-
marginTop : imgDim.top
+
});
});
-
 
});
});
-
+
</script> <!--slide show-->
-
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+
<!-- GA -->
-
_getImageDim : function( src ) {
+
<script type="text/javascript">
-
+
var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
-
var $img    = new Image();
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-
+
</script>
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-
+
try {
-
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-
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+
pageTracker._trackPageview();
-
r_w = c_h / c_w,
+
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-
+
-
i_w = $img.width,
+
-
i_h = $img.height,
+
-
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+
-
new_w, new_h, new_left, new_top;
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-
+
-
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-
+
-
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+
-
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+
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+
-
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+
-
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-
+
-
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+
-
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+
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+
-
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-
+
-
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-
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-
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+
-
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+
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+
-
+
-
},
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-
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+
-
+
-
// set the max-width of the slider elements to the one set in the plugin's options
+
-
// also, the width of each slider element will be 100% / total number of elements
+
-
this.$sliderElems.css({
+
-
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+
-
'width' : 100 / this.itemsCount + '%'
+
-
});
+
-
+
-
// set the max-width of the slider and show it
+
-
this.$sliderthumbs.css( 'max-width', this.options.thumbMaxWidth * this.itemsCount + 'px' ).show();
+
-
+
-
},
+
-
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+
-
+
-
var _self = this;
+
-
+
-
this.slideshow = setTimeout( function() {
+
-
+
-
var pos;
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-
+
-
( _self.current === _self.itemsCount - 1 ) ? pos = 0 : pos = _self.current + 1;
+
-
+
-
_self._slideTo( pos );
+
-
+
-
if( _self.options.autoplay ) {
+
-
+
-
_self._startSlideshow();
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-
+
-
}
+
-
+
-
}, this.options.slideshow_interval);
+
-
+
-
},
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-
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+
-
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+
-
+
-
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+
-
if( pos === this.current || this.isAnimating )
+
-
return false;
+
-
+
-
this.isAnimating = true;
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-
+
-
var $currentSlide = this.$imgItems.eq( this.current ),
+
-
$nextSlide = this.$imgItems.eq( pos ),
+
-
_self = this,
+
-
+
-
preCSS = {zIndex : 10},
+
-
animCSS = {opacity : 1};
+
-
+
-
// new slide will slide in from left or right side
+
-
if( this.options.animation === 'sides' ) {
+
-
+
-
preCSS.left = ( pos > this.current ) ? -1 * this.elWidth : this.elWidth;
+
-
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+
-
+
-
}
+
-
+
-
// titles animation
+
-
$nextSlide.find('div.ei-title > h2')
+
-
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+
-
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+
-
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+
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+
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+
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-
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-
+
-
$.when(
+
-
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$(this).show().css( 'opacity', 0 );
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+
-
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+
-
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-
// "sliding div" moves to new position
+
-
this.$sliderElem.stop().animate({
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-
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+
-
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+
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+
-
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+
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var _self = this;
+
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+
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+
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+
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// resize the images
+
-
_self._setImagesSize();
+
-
+
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// reset position of thumbs sliding div
+
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_self.$sliderElem.css( 'left', _self.$thumbs.eq( _self.current ).position().left );
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-
+
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+
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+
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+
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this.$thumbs.on( 'click.eislideshow', function( event ) {
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if( _self.options.autoplay ) {
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-
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-
clearTimeout( _self.slideshow );
+
-
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+
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+
-
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+
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+
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+
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+
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+
<!--project step js-->
-
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<script>
-
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-
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-
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-
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+
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this.each(function() {
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var instance = $.data( this, 'eislideshow' );
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if ( !instance ) {
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-
logError( "cannot call methods on eislideshow prior to initialization; " +
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return;
+
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-
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if ( !$.isFunction( instance[options] ) || options.charAt(0) === "_" ) {
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-
instance[ options ].apply( instance, args );
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else {
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this.each(function() {
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var instance = $.data( this, 'eislideshow' );
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-
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+
-
+
-
return this;
+
-
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-
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+
-
+
-
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+
-
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+
-
<script>
+
-
jQuery.easing['jswing'] = jQuery.easing['swing'];
+
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+
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jQuery.extend( jQuery.easing,
+
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{
+
-
def: 'easeOutQuad',
+
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swing: function (x, t, b, c, d) {
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Latest revision as of 13:25, 26 October 2012

Overview

Red tide detector: Facilitate small RNA as information processing media;
and gas vesicle as a novel output actuator...
Response to the overloading phosphate or nitrate when they exceeds warning limit...

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overview

Sensor

The sensors for next-generation detector of red tide!

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sensor

Model-Driven Design

Get specific directions for ternary system design from simulation...

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Home-modeling

Making decision through sRNA

The sophisticated 'CPU' of our red tide detector...
Engineering CPU for your own "synthia"!

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Home-decision-making-device

Floating Device

The output actuator of our red tide detector...
Gas vesicle as our floating device

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Oceanfloat and Oceanfeel
——a floating biosensor

Our Project

  • Part1
  • Part2
  • Part3
 

  • Sensor

    Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.

    much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.

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  • Decision-making Device

    Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.

    Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).

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  • Floating Device

    In our project, by transforming the buoyancy device BBa_K737010(designed by ourselves), the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.

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Human practice


Retrieved from "http://2012.igem.org/Team:OUC-China"