Team:Groningen/parts fail

From 2012.igem.org

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Restriction analysis (single cut)
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Restriction analysis (dual cut with EcoRI and PstI)
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The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is shorter than the expected size.
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The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is not a single band and shorter than the expected size.
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The restriction enzymes cut at illegal site(s)
The restriction enzymes cut at illegal site(s)
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                                        Gel Pic
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                                <img src=" " ></img>
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Revision as of 12:28, 26 September 2012





Failed Biobricks


List of failed BioBricks

BBa_K090403

Name: Gram-positive Shuttle Vector for Chromosomal Integration
Intended Purpose: Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol: Restriction analysis (dual cut with EcoRI and PstI)
Results: The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is not a single band and shorter than the expected size.



BBa_I742123

Name: Multi-host vector pTG262 converted to BioBrick vector
Intended Purpose: Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol: Restriction analysis (single cut) and restriction analysis (dual cut)
Results: The restriction enzymes cut at illegal site(s)
Gel Pic



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