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Team:Groningen/Sticker
From 2012.igem.org
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</html> | </html> | ||
| + | <html> | ||
| + | <head> | ||
| + | <style> | ||
| + | p{ | ||
| + | margin-left:150px; | ||
| + | font-size:12pt; | ||
| + | line-height:14pt; | ||
| + | margin-right:150px; | ||
| + | color:white; | ||
| + | } | ||
| + | p.marginleft{ | ||
| + | font-size:12pt; | ||
| + | line-height:14pt; | ||
| + | color:white; | ||
| + | margin-top:0px; | ||
| + | margin-bottom:38px; | ||
| + | margin-right:0px; | ||
| + | margin-left:150px; | ||
| + | margin-right:150px; | ||
| + | } | ||
| + | z2 { | ||
| + | font-size:18pt; | ||
| + | line-height:18pt; | ||
| + | color:white; | ||
| + | background-color: rgba(0,0,0,0.3); | ||
| + | } | ||
| + | z3 { | ||
| + | font-size:18pt; | ||
| + | line-height:18pt; | ||
| + | color:white; | ||
| + | background-color: rgba(0,0,0,0.3); | ||
| + | margin-left:150px; | ||
| + | } | ||
| + | z4 { | ||
| + | font-size:14pt; | ||
| + | font-weight: bold; | ||
| + | color:white; | ||
| + | } | ||
| + | ol.ref{ | ||
| + | list-style-type: decimal; | ||
| + | margin-left:170px; | ||
| + | margin-right:150px; | ||
| + | font-size:12pt; | ||
| + | color:white; | ||
| + | } | ||
| + | </style> | ||
| + | </head> | ||
| + | <z2>Design requirements</z2> | ||
| + | <p class="marginleft"> | ||
| + | <font color=#FF6700><b>Material</b></font> <br> | ||
| + | The material should not break to fast and should full fill human grip strength minimum of 40 pounds pinch and pressure. <br> | ||
| + | The product should be made of light, easy to handle material. <br> | ||
| + | The material needs to be not too expensive and durable.<br> | ||
| + | The volatiles and oxygen should penetrate throw the material.<br> | ||
| + | The material should fit in a meat box.<br> | ||
| + | The should not be toxic or getting toxic for the bacteria<br> | ||
| + | The material should handle at least 125 Celsius degree temperatuur.<br> | ||
| + | |||
| + | <br> | ||
| + | <font color=#FF6700><b>Measurements</b></font> <br> | ||
| + | Needs a feasible feedback for the human eye and should easy to understand for the customer. <br> | ||
| + | The product should keep the feasible feedback store in the material. <br> | ||
| + | <br> | ||
| + | <font color=#FF6700><b>Appearance</b></font> <br> | ||
| + | Product should be made of attractive colors and shape.<br> | ||
| + | <br> | ||
| + | <font color=#FF6700><b>Safety</b></font><br> | ||
| + | Material especially in the sticker, the bacteria’s should not go out of the sticker and not harm the environment and provide adequate support; and be reliable.<br> | ||
| + | <br> | ||
| + | <font color=#FF6700><b>Customer comfort</b></font> <br> | ||
| + | Easy to use the feasible feedback device. <br> | ||
| + | The product may provide different degrees of strength depending on the development of the customer strength. <br> | ||
| + | </p> | ||
| + | |||
| + | <z3>Develop design</z3> | ||
| + | <p class="marginleft"> | ||
| + | The development from the sticker the following strategy was used the PDCA (Plan Do Check Act) to develop a ultimate device that the customer and environment needs is involved in the project design.<br> | ||
| + | <br> | ||
| + | The following question where made: <br> | ||
| + | <br> | ||
| + | Who is your external customer?<br> | ||
| + | The customer that is against food spoilage and customers that are willing to buy it.<br> | ||
| + | <br> | ||
| + | What value do you deliver to that customer?<br> | ||
| + | A replacement for use by date and sell by date and new indicator for when meat is getting spoiled.<br> | ||
| + | <br> | ||
| + | Who, in you iGEM group, delivers that value?<br> | ||
| + | The design engineers and cooperation with the biologist to get input for the prototype design.<br> | ||
| + | <br> | ||
| + | How do they deliver that value?<br> | ||
| + | To do research and create a new indicator to indicate when meat get spoiled.<br> | ||
| + | </p> | ||
| + | |||
| + | <z3>Material</z3> | ||
| + | <p class="marginleft"> | ||
| + | Two different materials should be chosen carefully for our final product. In first term, the outer bag should be resistant enough to support the bacteria inside the sticker, strength and pressure that is applied by the customer. Its physical properties and characteristics do not have to be affected when to much pressure or deformation is applied on the sticker. It should be light and small for easy removing and placing in the meat bag. | ||
| + | In second term, the case should be made from a light material and the surface should be flat to avoid any risk for the user.<br> | ||
| + | <br> | ||
| + | Taking into account all these characteristics we considered three materials for the sticker: polyetheen (sanwich bag), Polyvinyl chloride (cling film) and Polymethylpentene (PMP) als commonly known as TPX. On the other hand, TPX was considered for the outer bag of the sticker. The reason was that all the requirements was for filled by this material. | ||
| + | In addition, a second material is needed inside the the sticker to separate the medium from the spores: The Polyvinyl chloride was to weak for the outer layer for the sticker. The strength and pressure that will be applied by the customer will break the sticker to fast and the sticker indication liquid will come accidentally come in the environment, what shouldn't happen for safety. For easy to break the inner compartment with medium in the sticker, this material had the best characteristics to full fill our second material needs. Not allot of pressure and strength is needed to break the inner compartment, just to give comfort to the customer when handling the sticker.<br> | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | <z4>Refences</z4> | ||
| + | <ol class="ref"> | ||
| + | <li>Krentsel B.A., Kissin Y.V., Kleiner V.I., Stotskaya S.S. Polymers and Copolymers of Higher a-Olefins, Hanser Publishers: New York, 1997.</li> | ||
| + | <li>H. C. Raine, J. Appl. Polym. Sci. 11, 39 (1969)</li> | ||
| + | <li>Mitsui Chemicals Co., Properties of Standard TPX Grades, 2004.</li> | ||
| + | <li>FDA CFR Title 21 Sec. 177.1520 Olefin polymers (C) 3.3b for TPX(4-methylpentene-1-based olefin copolymer).</li> | ||
| + | <li>Mathiowetz V, Kashman N, Volland G, Weber K, Dowe M, Rogers S (February 1985). "Grip and pinch strength: normative data for adults". Arch Phys Med Rehabil 66 (2): 69–74. PMID 3970660.</li> | ||
| + | </ol> | ||
| + | </p> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | <z3>Tools and supply</z3> | ||
| + | <p class="marginleft"> | ||
| + | - Sealboy type: 236 SBSA-2, Serie: 0070133017 to affix the TPX material and cling film in the setting 7.<br> | ||
| + | - Sanyo labo autoclave MLS-3020U (RUG Serial: 4495)to sterilize the TPX material.<br> | ||
| + | - Gas profile 1 SCS, Part: 6.103.000, series: 0213372 to work sterile during fill-up the sticker.<br> | ||
| + | - B-D Plastipak 21G 1<sup>1/2</sup> 40/8 NR2 2ml to fill up the sticker.<br> | ||
| + | - Cling film brand: Folia vershoudfolie, 50m width 29cm, 15 micrometer, oxygen permeability, fat- and waterproof. Inner compartment for the the sticker.<br> | ||
| + | - TPX X-44B#25 and X-44#50, Company: Mitsui Chemicals Co., Brand: TPX to use as outer layer from the sticker.<br> | ||
| + | - Luria Broth Brand: Lenox.<br> | ||
| + | </p> | ||
| + | |||
| + | <z3>Prototype</z3> | ||
| + | <p class="marginleft"> | ||
| + | The prototype the inner compartment is made with cling film with side affix of 1mm thickness the inner pack was filled with Luria broth and | ||
| + | </p> | ||
| + | |||
| + | <z3>Test</z3> | ||
| + | <p class="marginleft">text | ||
| + | </p> | ||
| + | |||
| + | <z3>Results</z3> | ||
| + | <p class="marginleft">text | ||
| + | </p> | ||
| + | |||
| + | </html> | ||
Revision as of 18:28, 22 September 2012
