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Team:Groningen/Notebook/Wetwork 8August2012
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Revision as of 13:45, 9 August 2012 by Nisa.purwanto (Talk | contribs)
Elbrich
Phusion PCR reaction with violacein DNA, BBa_K274002.
Nisa
Ligated fnr and lacI promoter with pigment(s) coding region and introduced the ligation product into E. coli DH5a
List of transformation:
- lacI promoter + amilCP (blue)
- lacI promoter + amilGFP (yellow)
- lacI promoter + lycopene (red)
- fnr promoter + amilCP
- fnr promoter + amilGFP
- fnr promoter + lycopene
- ) lacI promoter was induced by 0.2 and 0.5 mM IPTG
