Team:Groningen/Notebook/Wetwork 6August2012

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<A HREF="http://www.imik.org/wettelijke_context/Europese_hygienerichtlijn_en_microbiologische_criteria.pdf"><FONT COLOR=#ff6700>reference</FONT></A>
<A HREF="http://www.imik.org/wettelijke_context/Europese_hygienerichtlijn_en_microbiologische_criteria.pdf"><FONT COLOR=#ff6700>reference</FONT></A>
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Renske
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Primer design for positivie feedback loop (with Nisa)
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I PlacI
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a) Prefix + PLacI_Forward
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b) homology with pRe_F + PLacI_Rv
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c) homology with pLuxR + PLuxR_Rv
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II P_Activator
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a) homology pLac + PRE_F
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b) homology pLac _ pLuxR
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c) homology cII with pRE_R
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d) homology LuxI + pLUxR_R
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III Activator
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a) homology with pRE + CII_F
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b) homolgy with pLuxI + LuxI_F/ GFP + CII_R/GFP+LuxI_R
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IV GFP
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a) CII + GFP_F
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b) LuxI + GFP_R
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c) suffix _ GFP_R
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Revision as of 12:38, 21 September 2012






Tom

PCR alsT (150, 250, 300, 500) + GFP construct with taq-polymerase. Restriction analysis for alsT (150, 250, 300, 500) + GFP with ecoRI.

Renske

Counted TAMC plates + analyzed results:












reference


Renske

Primer design for positivie feedback loop (with Nisa)

I PlacI a) Prefix + PLacI_Forward b) homology with pRe_F + PLacI_Rv c) homology with pLuxR + PLuxR_Rv

II P_Activator a) homology pLac + PRE_F b) homology pLac _ pLuxR c) homology cII with pRE_R d) homology LuxI + pLUxR_R

III Activator a) homology with pRE + CII_F b) homolgy with pLuxI + LuxI_F/ GFP + CII_R/GFP+LuxI_R

IV GFP a) CII + GFP_F b) LuxI + GFP_R c) suffix _ GFP_R

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