Team:Groningen/Notebook/Wetwork 3July2012
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Experimental duplo of 02/07. Cells harvested and kept in -80. | Experimental duplo of 02/07. Cells harvested and kept in -80. | ||
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+ | Nisa/Emeraldo | ||
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+ | 1.) Linearization of: BBa_K274100 (red pigment), BBa_K274002 (violacein-violet pigment), BBa_K090403 (amyE integration plasmid), BBa_R0062 (luxR promoter), BBa_J37034 (luxI, GFP added). All of them are in correct length except the integration plasmid (expected) | ||
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+ | 2.) B. subtilis transformation using BBa_K090403 and BBa_I742123 by Ruud from Molgen group (to compare with our work) |
Revision as of 17:20, 8 July 2012
Micro-array harvest experiment
Experimental duplo of 02/07. Cells harvested and kept in -80.
Nisa/Emeraldo
1.) Linearization of: BBa_K274100 (red pigment), BBa_K274002 (violacein-violet pigment), BBa_K090403 (amyE integration plasmid), BBa_R0062 (luxR promoter), BBa_J37034 (luxI, GFP added). All of them are in correct length except the integration plasmid (expected)
2.) B. subtilis transformation using BBa_K090403 and BBa_I742123 by Ruud from Molgen group (to compare with our work)