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Team:Groningen/Notebook/Wetwork 28August2012
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Redo the ligation of Fnr promotor and alsT300 promotor with lycopene and GFP in Psac. | Redo the ligation of Fnr promotor and alsT300 promotor with lycopene and GFP in Psac. | ||
Result: Incubation temperature of the ligation was too high resulting in no colonies after transformation. | Result: Incubation temperature of the ligation was too high resulting in no colonies after transformation. | ||
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| + | Tom/Arjan | ||
5 overnight cultures of each previous transformation, 26August2012, were incubated. | 5 overnight cultures of each previous transformation, 26August2012, were incubated. | ||
Result: All the colonies contained empty Psac plasmid. | Result: All the colonies contained empty Psac plasmid. | ||
Revision as of 13:00, 30 August 2012
Tom
Redo the ligation of Fnr promotor and alsT300 promotor with lycopene and GFP in Psac.
Result: Incubation temperature of the ligation was too high resulting in no colonies after transformation.
Tom/Arjan
5 overnight cultures of each previous transformation, 26August2012, were incubated.
Result: All the colonies contained empty Psac plasmid.
