Team:Groningen/Notebook/Wetwork 20June2012

From 2012.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 1: Line 1:
{{HeaderGroningen2012}}
{{HeaderGroningen2012}}
 +
<html>
 +
<head>
 +
<style type="text/css">
 +
p.margin
 +
{
 +
font-size:12pt;
 +
line-height:14pt;
 +
color:white;
 +
margin-top:0px;
 +
margin-bottom:20px;
 +
margin-left:150px;
 +
margin-right:250px;
 +
}
-
Bacillus 168 strain provided by Jan-Willem Veening. Grown overnight on LB agar plate at 37C.
 
 +
</style>
 +
</head>
 +
 +
<body>
 +
 +
<p class="margin">
 +
<br><br><br>
 +
Bacillus 168 strain provided by Jan-Willem Veening. Grown overnight on LB agar plate at 37C.
 +
<br><br>
Plasmid isolation of bacillus backbone for chromosomal integration (BBa_K090403, size: 5632 bp) and terminator biobricks (BBa_B0020 and BBa_B0012, both size: 2058 bp) using the Roche High Pure Plasmid Isolation Kit.
Plasmid isolation of bacillus backbone for chromosomal integration (BBa_K090403, size: 5632 bp) and terminator biobricks (BBa_B0020 and BBa_B0012, both size: 2058 bp) using the Roche High Pure Plasmid Isolation Kit.
 +
<br><br>
 +
 +
Plasmid concentrations after isolation: <br>
 +
> BBa_K090403: 50.8 ng/uL, 260/280 = 1.85 <br>
-
Plasmid concentrations after isolation:  
+
> BBa_B0020: 93.5 ng/uL, 260/280 = 1.84 <br>
-
> BBa_K090403: 50.8 ng/uL, 260/280 = 1.85
+
> BBa_ B0012: 60.0 ng/uL, 260/280 = 1.93 <br>
-
> BBa_B0020: 93.5 ng/uL, 260/280 = 1.84
+
<br>
 +
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
 +
</p><br><br>
 +
</body>
 +
</html>
-
> BBa_ B0012: 60.0 ng/uL, 260/280 = 1.93
+
{{Template:SponsorsGroningen2012}}

Latest revision as of 16:48, 25 September 2012







Bacillus 168 strain provided by Jan-Willem Veening. Grown overnight on LB agar plate at 37C.

Plasmid isolation of bacillus backbone for chromosomal integration (BBa_K090403, size: 5632 bp) and terminator biobricks (BBa_B0020 and BBa_B0012, both size: 2058 bp) using the Roche High Pure Plasmid Isolation Kit.

Plasmid concentrations after isolation:
> BBa_K090403: 50.8 ng/uL, 260/280 = 1.85
> BBa_B0020: 93.5 ng/uL, 260/280 = 1.84
> BBa_ B0012: 60.0 ng/uL, 260/280 = 1.93

Back to notebook