Team:Groningen/Notebook/Wetwork 17August2012

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Tom
 
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PCRed the different promotors alsT 150, 250, 300, 500 and Fnr to GFP.
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Result: None of the pcr products contained the desired product. It is not sure if this is due to wrong primers or not approprate working taq polymerase.
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Tom <br>
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Emeraldo
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PCRed the different promotors alsT 150, 250, 300, 500 and Fnr to GFP. <br>
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Cut the pSac-Cm and purified terminator PCR product with EcoRI and HindIII. Cut pSac-Cm was run on the gel and cut at the correct cut size and purified from the gel. Ligation of cut pSac-Cm with cut terminator and then transformation of E.coli DH5alpha with the ligation product.
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Result: None of the pcr products contained the desired product. It is not sure if this is due to wrong primers or not appropriate working taq polymerase. <br>
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Emeraldo <br>
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Cut the pSac-Cm and purified terminator PCR product with EcoRI and HindIII. Cut pSac-Cm was run on the gel and cut at the correct cut size and purified from the gel. Ligation of cut pSac-Cm with cut terminator and then transformation of E.coli DH5alpha with the ligation product. <br>
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Nisa<br>
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Plasmid isolation of the transformans<br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 13:40, 26 September 2012







Tom

PCRed the different promotors alsT 150, 250, 300, 500 and Fnr to GFP.

Result: None of the pcr products contained the desired product. It is not sure if this is due to wrong primers or not appropriate working taq polymerase.


Emeraldo

Cut the pSac-Cm and purified terminator PCR product with EcoRI and HindIII. Cut pSac-Cm was run on the gel and cut at the correct cut size and purified from the gel. Ligation of cut pSac-Cm with cut terminator and then transformation of E.coli DH5alpha with the ligation product.


Nisa
Plasmid isolation of the transformans

Back to notebook

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