Nisa

PCR of GFP (optimized for B. subtilis) with the recieved primers T melting Fwd= 57C T melting Rev = 61C Therefore, make a gradient from 55C-65C (55, 57, 59,61,63,65)

Ligation primer

idea: ligate mulitple fragments of DNA without T4 ligase, but using the PCR method.

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