Team:Groningen/Notebook/Wetwork 16July2012

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Nisa
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PCR of GFP with the recieved primers
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<body><br><br><br>
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T melting Fwd= 57C  
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T melting Rev = 61C
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Nisa<br>
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Therefore, make a gradient from 55-65C
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<br>
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PCR of GFP (optimized for B. subtilis) with the recieved primers<br>
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T melting Fwd= 57C <br>
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T melting Rev = 61C<br>
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Therefore, make a gradient from 55C-65C (55, 57, 59,61,63,65)<br>
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<br>
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<br>
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Ligation primer<br>
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<br>
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idea: ligate mulitple fragments of DNA without T4 ligase, but using the PCR method.<br>
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<br>
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<br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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{{Template:SponsorsGroningen2012}}
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Ligation primer
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idea: ligate mulitple fragments of DNA without T4 ligase, but using PCR
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Latest revision as of 21:09, 25 September 2012







Nisa

PCR of GFP (optimized for B. subtilis) with the recieved primers
T melting Fwd= 57C
T melting Rev = 61C
Therefore, make a gradient from 55C-65C (55, 57, 59,61,63,65)


Ligation primer

idea: ligate mulitple fragments of DNA without T4 ligase, but using the PCR method.


Back to notebook



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