"
Team:Groningen/Notebook/Wetwork 15August2012
From 2012.igem.org
Revision as of 13:02, 26 September 2012 by Nisa.purwanto (Talk | contribs)
Tom
Ligation of alsT(150, 250, 300, 500) with GFP and psb1c3 and pfu promotor with GFP and psb1c3. Result: Transformation in E.coli was succesfull.
PCR of GFP with preffix and suffix and PCR of GFP that can couple to pfu. Result: only PCR product: GFP preffix suffix was succesfull.
Emeraldo Sub-culturing the transformats colonies on LB agar plates
Nisa Promoters-pigment cloning into pSB1C3. Molarity ratio plasmid:DNA insert = 1:3. Promoter used in this step: fnr and lacI Pigment used in this step: lycopene, amilCP, amilGFP Ligation was done for 2 hours in room temperature before continue to E. coli transformation.
Our sponsors:
