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Team:Groningen/Data page
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| - | <li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818000" target=_blank>BBa_K818000</a> Integration vector for <i>Bacillus subtilis</i>< | + | <li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818000" target=_blank>BBa_K818000</a> <b>Integration vector for <i>Bacillus subtilis</i></b> - This vector is designed to integrate into the chromosome of <i>Bacillus subtilis</i> at the SacA locus and replicate in <i>Escherichia coli</i>. The vector is derived from the vector <i>psac-cm</i> (<a href=http://www.sciencedirect.com/science/article/pii/S0147619X04000332 target =_blank>Middleton et al., 2004</a>). Our vector contains the biobrick compatible restriction sites, a terminator (BBa_B0015) after the suffixes sequences and the sequence for RFP (BBa_J04450) in its multiple cloning site (MCS).<br></li> |
| - | This vector is designed to integrate into the chromosome of <i>Bacillus subtilis</i> at the SacA locus and replicate in <i>Escherichia coli</i>. The vector is derived from the vector <i>psac-cm</i> (<a href=http://www.sciencedirect.com/science/article/pii/S0147619X04000332 target =_blank>Middleton et al., 2004</a>). Our vector contains the biobrick compatible restriction sites, a terminator (BBa_B0015) after the suffixes sequences and the sequence for RFP (BBa_J04450) in its multiple cloning site (MCS).<br></li> | + | |
<li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818600" target=_blank>BBa_K818600</a> Rotting meat sensor system<br> | <li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818600" target=_blank>BBa_K818600</a> Rotting meat sensor system<br> | ||
This construct contains the native <i>Bacillus subtilis</i> promoter PsboA (BBa_K818100) connected to the yellow chromoprotein amilGFP (BBa_K592010) with RBS (B0034). PsboA is strongly activated at the presence of volatiles from rotten meat when <i>Bacillus subtilis</i> is in exponential growth. The system can produce a visible yellow output in six hours.<br></li> | This construct contains the native <i>Bacillus subtilis</i> promoter PsboA (BBa_K818100) connected to the yellow chromoprotein amilGFP (BBa_K592010) with RBS (B0034). PsboA is strongly activated at the presence of volatiles from rotten meat when <i>Bacillus subtilis</i> is in exponential growth. The system can produce a visible yellow output in six hours.<br></li> | ||
Revision as of 13:10, 26 October 2012
Data For Our Favorite New Parts
BBa_K818000 Integration vector for Bacillus subtilis - This vector is designed to integrate into the chromosome of Bacillus subtilis at the SacA locus and replicate in Escherichia coli. The vector is derived from the vector psac-cm (Middleton et al., 2004). Our vector contains the biobrick compatible restriction sites, a terminator (BBa_B0015) after the suffixes sequences and the sequence for RFP (BBa_J04450) in its multiple cloning site (MCS).- BBa_K818600 Rotting meat sensor system
This construct contains the native Bacillus subtilis promoter PsboA (BBa_K818100) connected to the yellow chromoprotein amilGFP (BBa_K592010) with RBS (B0034). PsboA is strongly activated at the presence of volatiles from rotten meat when Bacillus subtilis is in exponential growth. The system can produce a visible yellow output in six hours.
Data For Pre-existing Parts
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We've Also Characterized the Following Parts
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