Team:Goettingen/week4-3

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#3 Chemoreceptor Library - 4th Week

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V05_22


Preparation of plates, solutions
  • Experiment:
    Preparation of 0.1 M IPTG solution. Preparation of LB-Amp-Xgal/IPTG agar plates.


V05_23


Amplifcation of the tar gene
  • Experiment:
    PCR was performed with isolated genomic DNA from DH10B, forward and reverse primers of tar and using Pfu polymerase. Clean up with peqGOLD Gelextraction Kit (Peqlab). A band at about 1.6 kb was detected. Amplification of tar was succhesfull.


V05_25


Preparation of chemocompetent cells, LB-media and agarplates. Transformation of BioBrick promoters
  • Experiment:
    Chemocompetent cells and media were prepared like described here. For transformation of BioBrick promoters see Labbook group #2.


V05_27


Preparation of DH10B with promoter constructs
  • Experiment:
    Over-night culture of DH10B cells. DNA was prepped using peqGOLD MiniPrep Kit (Peqlab).


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