Team:Fudan Lux/Notebook

We summarized our recent brain storm result. At last we chose to make a light-signal oscillator in E.coli and possibilities to use nanotubes. The first thing for the next week is to find an efficient light sensor and a stable lighting part.

Discuss the establishment of the gene circuits.

Get lux without operon and promoter from k325909 by PCR. We used longPCR Enzyme and Overlapping PCR.

Measure the growth curve of dH5a transformed by lux with araBAD (k325909)

Transform tetR (C0040) from the kit. Transfer araBAD (I0500) and tetR (C0040) with RBS into pSB1C3 by 3A ligation. Transform it into dH5a.

Transfer tetR with promoter and RFP with ptetO as promoter (J61002) into pSB1C3 by 3A ligation. Transform it into dH5a.

Sorry to find the ligation is failed. But fortunately, we decide to change the reporter into GFP (I13522). Transform the plasmid from the kit into dH5a.

Design protein composed by lov domain and modified helix turn helix (abbr. lov-HTH).

Design protein composed by lov domain and tetR (abbr. lov-tetR).

Send the synthetic orders to the gene synthesis company.

Half of our members went to Tianmu Mount, Zhejiang Province. They spent a week there to participate a fieldwork organized by the Life Science Institute. During the fieldwork, they paid a lot of attention to a China’s specific firefly, Qiongyuying. They observed the behavior of Qiongyuying, especially the synchrony.

Our instructor and four of our members went to meet Richard J Roberts after the reward ceremony of COMBREX, a project to accelerate the functional annotation of prokaryotic genomes. They talked for about an hour and we presented our project to him. Richard advised that we should make our project more practical and functional.

After negotiating with the principal of volunteer teaching program in our institute, one of our members, with all members of Fudan_Lux’s hope and responsibility on his shoulders, went to Shangrao, Jiangxi Province to do the volunteer teaching.

Make an appointment with Li Jin, Hongyan Wang etc. to show our projects.

Receive lov-HTH in nonstandard vector.

Design primers to make clones of lov-HTH, and transfer lov-HTH with double terminators into pSB1C3.

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, commodo vitae, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. Donec non enim in turpis pulvinar facilisis. Ut felis. Praesent dapibus, neque id cursus faucibus, tortor neque egestas augue, eu vulputate magna eros eu erat. Aliquam erat volutpat. Nam dui mi, tincidunt quis, accumsan porttitor, facilisis luctus, metus

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, commodo vitae, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. Donec non enim in turpis pulvinar facilisis. Ut felis. Praesent dapibus, neque id cursus faucibus, tortor neque egestas augue, eu vulputate magna eros eu erat. Aliquam erat volutpat. Nam dui mi, tincidunt quis, accumsan porttitor, facilisis luctus, metus

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, commodo vitae, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. Donec non enim in turpis pulvinar facilisis. Ut felis. Praesent dapibus, neque id cursus faucibus, tortor neque egestas augue, eu vulputate magna eros eu erat. Aliquam erat volutpat. Nam dui mi, tincidunt quis, accumsan porttitor, facilisis luctus, metus

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, commodo vitae, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt cond