Revision as of 21:32, 19 August 2012

Labjournal

Team Site Directed Mutagenesis: Plasmid-isolations of plated colonies
Team Cellulose Binding Domain: Redesigned the primer-sequences another time giving the CBDs a few AS more in than the Protein-BLAST said. CBDcex: 4 AS N-terminal, 2 C-terminal. CBDclos: 2 N-terminal (starting with a natural ATG) and 2 AS C-terminal.
- Prepared BBa_392014 for Sequencing

Team Site Directed Mutagenesis: More plasmid-isolations, test-restriktions and colony-pickings.

Team Wiki: This morning we met for taking a new picture of our group and individual pictures of everyone. Check out our beautiful team members here.

Team Site Directed Mutagenesis: Transformed T.vers_243_III_1161 and X.camp._2K_2 V_3633 in XL1 Blue and plated it on Ampicillin- and CM-selection-agar.
Team Cellulose Binding Domain: Transformed the plasmids p714_cex and 570_clos we got from the fermantationgroup in KRX an plated them on Kanamycin-selectionagar.

Team Site Directed Mutagenesis: Plated four isolated colonies from T.vers_243_III_1161 and X.camp._2K_2 V_3633 per petri dish on seperated selection-agar-dishes

Team Cellulose Binding Domain: Since there were only a few colonies on the p570 selection-agar-dish, I picked one, resuspended it in 100 µL destilled water and plated it on an new Kanamycin-selection-agar-dish
- plasmid-isolation of p714

Team Site Directed Mutagenesis: Plasmidisolations of X.camp.2K_2_3633 (I-IV) and T.vers_243_III_1161 (I-IV)
- Test-Restriktion of X.camp.2K_2_3633 (I-IV) and T.vers_243_III_1161 (I-IV) X.camp III + IV good; T.vers, no positiv, since the 1150 band should at least rise to 1550
- Picked two additional colonies of e.coli_2307 and plated them on CM
- Prepared X.camp.2K_2_3633 III + IV for sequencing
Team Cellulose Binding Domain: Transformation of BBa_I13522 + pSB1A2 in KRX and plating it on AMP-Selection-Agar
- Plasmidisolations of p570
- PCR of CBDcex (417 bp) and CBDclos (369 bp)
  - Test-Gelelectrophoresis: Got the bands that should be there!
  - PCR Clean up (no Gelelectrophoresis)
- Restriktion of PCR-products and pSB1C3_RFP with XbaI and PstI.

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 ===Friday August 3rd===
-* '''Team Site Directed Mutagenesis:''' More Testrestriktions and get a few Plasmids ready for sequencing
+* '''Team Site Directed Mutagenesis:''' Transformed ''T.vers''_243_III_1161 and ''X.camp.''_2K_2 V_3633 in XL1 Blue and plated it on Ampicillin- and CM-selection-agar.
 * '''Team Cellulose Binding Domain:''' Transformed the plasmids p714_cex and 570_clos we got from the fermantationgroup in KRX an plated them on Kanamycin-selectionagar.