Revision as of 16:49, 23 September 2012

Construct

Our construct idea is simple: production of pigment under the regulation of rotten-meat reactive promoter. When the Bacillus subtilis senses the volatiles from the rotten meat, the rotten meat promoter becomes active thus allowing the production of downstream genes. We put pigment genes available downstream of the promoter so that the pigment would be produced when the promoter is activated.

We use our Bacillus subtilis backbone (BBa_K818000) that has sacA and chloramphenicol resistance gene for chromosomal integration and transformants antibiotic screening. This backbone also has E. coli origin of replication, so it can be amplified inside E. coli.

There are several idea to improve our current construct, such as: multi-colored pigment system and fine tuning of the pigment production.

Multi-colored Pigment System

Fine Tuning of Pigment Production

Team:Groningen/PBADmeat

From 2012.igem.org

Revision as of 16:49, 23 September 2012

Our sponsors:

@@ Line 20: / Line 20: @@
                          background-color: transparent;
                          background-color: rgba(0,0,0,0.3);
-                         padding: 10px ;
+                         padding: 5px ;
                          margin: 30px auto;
                          color: #FFF;
@@ Line 32: / Line 32: @@
                          background-color: transparent;
                          background-color: rgba(0,0,0,0.3);
-                         margin: -30px auto;
+                         margin: 0px auto;
                          color: #FFF;
                       }
@@ Line 47: / Line 47: @@
 </html>
-<html>
 <html>
 <head>
 <style>
-body {
-			font-family: Helvetica, Arial, sans-serif;
-                }
-		stickerP {
-			font-size:12pt;
-                        line-height:12pt;
-                        color:white;
-		}
-                .sticker1 {
-                        width: 100px;
-			text-align: center;
-                        background: #000;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        padding: 10px ;
-                        margin: 0px auto;
-                        color: #FFF;
-					}
-                .sticker2 {
-			left: 50px;
-			top:300px;
-			position:absolute;
-                        width: 120px;
-			text-align: center;
-                        background: #000;
-			padding-right:10px;
-			padding-left:10px;
-                        margin: 0 auto;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        color: #FFF;
-					}
-</style>
-<div class="sticker2">
-<div class="sticker1">
-<stickerP ><A HREF="https://2012.igem.org/Team:Groningen/rotting"><FONT COLOR=#ffffff>sticker</FONT></A></stickerP>
-</div>
-</div>
-</body>
-</head>
-</html>
-<html>
-<head>
-<style>
-body {
-			font-family: Helvetica, Arial, sans-serif;
-                }
-		GerminationP {
-			font-size:12pt;
-                        line-height:12pt;
-                        color:white;
-		}
-                .Germination1 {
-                        width: 100px;
-			text-align: center;
-                        background: #000;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        padding: 10px ;
-                        margin: 0px auto;
-                        color: #FFF;
-					}
-                .Germination2 {
-			left: 200px;
-			top:300px;
-			position:absolute;
-                        width: 120px;
-			text-align: center;
-                        background: #000;
-			padding-right:10px;
-			padding-left:10px;
-                        margin: 0 auto;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        color: #FFF;
-					}
-</style>
-<div class="Germination2">
-<div class="Germination1">
-<GerminationP ><A HREF="https://2012.igem.org/Team:Groningen/germinationandsporulation"><FONT COLOR=#ffffff>germination</FONT></A></GerminationP>
-</div>
-</div>
-</body>
-</head>
-</html>
-<html>
-<head>
-<style>
-body {
-			font-family: Helvetica, Arial, sans-serif;
-                }
-		PBADmeat1P {
-			font-size:12pt;
-                        line-height:12pt;
-                        color:white;
-		}
-                .PBADmeat11 {
-                        width: 100px;
-			text-align: center;
-                        background: #000;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        padding: 10px ;
-                        margin: 0px auto;
-                        color: #FFF;
-					}
-                .PBADmeat12 {
-			left: 350px;
-			top:300px;
-			position:absolute;
-                        width: 120px;
-			text-align: center;
-                        background: #000;
-			padding-right:10px;
-			padding-left:10px;
-                        margin: 0 auto;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        color: #FFF;
-					}
-</style>
-<div class="PBADmeat12">
-<div class="PBADmeat11">
-<PBADmeat1P ><A HREF="https://2012.igem.org/Team:Groningen/volatiles"><FONT COLOR=#ffffff>volatiles</FONT></A></PBADmeat1>
-</div>
-</div>
-</body>
-</head>
-</html>
-<html>
-<head>
-<style>
-body {
-			font-family: Helvetica, Arial, sans-serif;
-                }
-		PBADmeat2P {
-			font-size:12pt;
-                        line-height:12pt;
-                        color:white;
-		}
-                .PBADmeat21 {
-                        width: 100px;
-			text-align: center;
-                        background: #000;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        padding: 10px ;
-                        margin: 0px auto;
-                        color: #FFF;
-					}
-                .PBADmeat22 {
-			left: 500px;
-			top:300px;
-			position:absolute;
-                        width: 120px;
-			text-align: center;
-                        background: #000;
-			padding-right:10px;
-			padding-left:10px;
-                        margin: 0 auto;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        color: #FFF;
-					}
-</style>
-<div class="PBADmeat22">
-<div class="PBADmeat21">
-<PBADmeat2P ><A HREF="https://2012.igem.org/Team:Groningen/identication"><FONT COLOR=#ffffff>identification</FONT></A></PBADmeat2P>
-</div>
-</div>
-</body>
-</head>
-</html>
-<html>
-<head>
-<style>
-body {
-			font-family: Helvetica, Arial, sans-serif;
-                }
-		PigmentsP {
-			font-size:12pt;
-                        line-height:12pt;
-                        color:white;
-		}
-                .Pigments1 {
-                        width: 100px;
-			text-align: center;
-                        background: #000;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        padding: 10px ;
-                        margin: 0px auto;
-                        color: #FFF;
-					}
-                .Pigments2 {
-			left: 650px;
-			top:300px;
-			position:absolute;
-                        width: 120px;
-			text-align: center;
-                        background: #000;
-			padding-right:10px;
-			padding-left:10px;
-                        margin: 0 auto;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        color: #FFF;
-					}
-</style>
-<div class="Pigments2">
-<div class="Pigments1">
-<PigmentsP ><A HREF="https://2012.igem.org/Team:Groningen/PBADmeat"><FONT COLOR=#ffffff>pigment</FONT></A></Pigments>
-</div>
-</div>
-</body>
-</head>
-</html>
-<html>
-<head>
-<style type="text/css">
-body {
-			font-family: Helvetica, Arial, sans-serif;
-                }
-		ConstructP {
-			font-size:12pt;
-                        line-height:12pt;
-                        color:white;
-		}
-                .Construct1 {
-                        width: 100px;
-			text-align: center;
-                        background: #000;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        padding: 10px ;
-                        margin: 0px auto;
-                        color: #FFF;
-					}
-                .Construct2 {
-			left: 800px;
-			top:300px;
-			position:absolute;
-                        width: 120px;
-			text-align: center;
-                        background: #000;
-			padding-right:10px;
-			padding-left:10px;
-                        margin: 0 auto;
-			background-color: transparent;
-			background-color: rgba(0,0,0,0.3);
-                        color: #FFF;
-					}
 p.margin
 {
@@ Line 367: / Line 78: @@
 </style>
-<div class="Construct2">
-<div class="Construct1">
-<ConstructP ><A HREF="https://2012.igem.org/Team:Groningen/pigmentproduction"><FONT COLOR=#ffffff>construct</FONT></A></ConstructP>
-</div>
-</div>
 <p class="margin">
 Our construct idea is simple: production of pigment under the regulation of rotten-meat reactive promoter. When the Bacillus subtilis senses the volatiles from the rotten meat, the rotten meat promoter becomes active thus allowing the production of downstream genes. We put pigment genes available downstream of the promoter so that the pigment would be produced when the promoter is activated.<br>
@@ Line 383: / Line 89: @@
 </p>
 <br><br><br>
-<div class="cte">
-<div class="ctd">
-<z1>Future Plan</z1>
-</div>
-</div>
 <br><br><br>
 <p class="margin">