Team:Groningen/Notebook/Wetwork 1September2012
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Miniprep of the overnight cultured transformed E.coli cells with the BioBricks from Germany. Names of the Biobricks that were used: BBa_K823021,BBa_K823023,BBa_K823024,BBa_K823026. NanoDrop concentrations were all higher than 120,5 ng/ul. | Miniprep of the overnight cultured transformed E.coli cells with the BioBricks from Germany. Names of the Biobricks that were used: BBa_K823021,BBa_K823023,BBa_K823024,BBa_K823026. NanoDrop concentrations were all higher than 120,5 ng/ul. | ||
- | BBa_K823024 was restricted with EcoR1, Pst1, XbaI, SpeI and the sizes were checked on gel. | + | To control the cutting sites of the Bricks, BBa_K823024 was restricted with EcoR1, Pst1, XbaI, SpeI and the sizes were checked on gel. |
Revision as of 14:33, 1 September 2012
Elbrich
Miniprep of the overnight cultured transformed E.coli cells with the BioBricks from Germany. Names of the Biobricks that were used: BBa_K823021,BBa_K823023,BBa_K823024,BBa_K823026. NanoDrop concentrations were all higher than 120,5 ng/ul.
To control the cutting sites of the Bricks, BBa_K823024 was restricted with EcoR1, Pst1, XbaI, SpeI and the sizes were checked on gel.