Team:Groningen/Sticker
From 2012.igem.org
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</body> | </body> | ||
+ | </html> | ||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
+ | body { | ||
+ | font-family: Helvetica, Arial, sans-serif; | ||
+ | |||
+ | } | ||
+ | stickerP { | ||
+ | font-size:12pt; | ||
+ | line-height:12pt; | ||
+ | color:white; | ||
+ | } | ||
+ | .sticker1 { | ||
+ | width: 110px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .sticker2 { | ||
+ | left: 340px; | ||
+ | top:600px; | ||
+ | position:absolute; | ||
+ | width: 130px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
+ | |||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <div class="sticker2"> | ||
+ | <div class="sticker1"> | ||
+ | <stickerP ><A HREF="https://2012.igem.org/Team:Groningen/Sticker" TARGET="_blank"><FONT COLOR=#ffffff>Sticker</FONT></A></stickerP> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
+ | |||
+ | body { | ||
+ | font-family: Helvetica, Arial, sans-serif; | ||
+ | |||
+ | } | ||
+ | GerminationP { | ||
+ | font-size:12pt; | ||
+ | line-height:12pt; | ||
+ | color:white; | ||
+ | } | ||
+ | .Germination1 { | ||
+ | width: 110px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .Germination2 { | ||
+ | left: 540px; | ||
+ | top:600px; | ||
+ | position:absolute; | ||
+ | width: 130px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
+ | |||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <div class="Germination2"> | ||
+ | <div class="Germination1"> | ||
+ | <GerminationP ><A HREF="https://2012.igem.org/Team:Groningen/Germination" TARGET="_blank"><FONT COLOR=#ffffff>Germination</FONT></A></GerminationP> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | |||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
+ | |||
+ | body { | ||
+ | font-family: Helvetica, Arial, sans-serif; | ||
+ | |||
+ | } | ||
+ | PBADmeat1P { | ||
+ | font-size:12pt; | ||
+ | line-height:12pt; | ||
+ | color:white; | ||
+ | } | ||
+ | .PBADmeat11 { | ||
+ | width: 110px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .PBADmeat12 { | ||
+ | left: 340px; | ||
+ | top:640px; | ||
+ | position:absolute; | ||
+ | width: 130px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
+ | |||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <div class="PBADmeat12"> | ||
+ | <div class="PBADmeat11"> | ||
+ | <PBADmeat1P ><A HREF="https://2012.igem.org/Team:Groningen/PBADmeat1" TARGET="_blank"><FONT COLOR=#ffffff>PBADmeat1</FONT></A></PBADmeat1> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
+ | |||
+ | body { | ||
+ | font-family: Helvetica, Arial, sans-serif; | ||
+ | |||
+ | } | ||
+ | PBADmeat2P { | ||
+ | font-size:12pt; | ||
+ | line-height:12pt; | ||
+ | color:white; | ||
+ | } | ||
+ | .PBADmeat21 { | ||
+ | width: 110px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .PBADmeat22 { | ||
+ | left: 540px; | ||
+ | top:640px; | ||
+ | position:absolute; | ||
+ | width: 130px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
+ | |||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <div class="PBADmeat22"> | ||
+ | <div class="PBADmeat21"> | ||
+ | <PBADmeat2P ><A HREF="https://2012.igem.org/Team:Groningen/PBADmeat2" TARGET="_blank"><FONT COLOR=#ffffff>PBADmeat2</FONT></A></PBADmeat2P> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
+ | |||
+ | body { | ||
+ | font-family: Helvetica, Arial, sans-serif; | ||
+ | |||
+ | } | ||
+ | PigmentsP { | ||
+ | font-size:12pt; | ||
+ | line-height:12pt; | ||
+ | color:white; | ||
+ | } | ||
+ | .Pigments1 { | ||
+ | width: 110px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .Pigments2 { | ||
+ | left: 340px; | ||
+ | top:680px; | ||
+ | position:absolute; | ||
+ | width: 130px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
+ | |||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <div class="Pigments2"> | ||
+ | <div class="Pigments1"> | ||
+ | <PigmentsP ><A HREF="https://2012.igem.org/Team:Groningen/Pigments" TARGET="_blank"><FONT COLOR=#ffffff>Pigments</FONT></A></Pigments> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
+ | |||
+ | body { | ||
+ | font-family: Helvetica, Arial, sans-serif; | ||
+ | |||
+ | } | ||
+ | DeathP { | ||
+ | font-size:12pt; | ||
+ | line-height:12pt; | ||
+ | color:white; | ||
+ | } | ||
+ | .Death1 { | ||
+ | width: 110px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .Death2 { | ||
+ | left: 540px; | ||
+ | top:680px; | ||
+ | position:absolute; | ||
+ | width: 130px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
+ | |||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <div class="Death2"> | ||
+ | <div class="Death1"> | ||
+ | <DeathP ><A HREF="https://2012.igem.org/Team:Groningen/Death" TARGET="_blank"><FONT COLOR=#ffffff>Death</FONT></A></DeathP> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
</html> | </html> | ||
Revision as of 13:58, 30 August 2012
The spores of Bacillus subtilis will be contained in a compartment which is impermeable to bacteria and liquid, but can still let through volatiles. Metabolites, water, and alanine should be separated from the spores by a breakable membrane. Mixing these compounds will cause germination.
The material we use for our sticker is TPX®, or polymethylpentene. This polymer is available as thin, transparent sheets. The advantage is that it is relatively cheap, strong, and capable of letting through volatiles. The radius of the pores in TPX® is 0.5 µm, which is 500x larger than the average badmeat-volatile, but still small enough to keep liquid and bacteria in (see the figure below).
Comparison of the size of the TPX pores, volatiles and Bacillus subtilis.