Team:Fudan Lux/nanotube

From 2012.igem.org

(Difference between revisions)
 
(31 intermediate revisions not shown)
Line 128: Line 128:
<!-- wrapper-header -->
<!-- wrapper-header -->
<div class="wrapper">
<div class="wrapper">
-
<a href="http://luiszuno.com/themes/nova/index.html"><img id="logo" src="https://static.igem.org/mediawiki/2012/5/5d/Logo_64px.png" alt="Nova"></a>
+
<a href="https://2012.igem.org/Team:Fudan_Lux"><img id="logo" src="https://static.igem.org/mediawiki/2012/5/5d/Logo_64px.png" alt="Nova"></a>
  <!-- search -->
  <!-- search -->
     <div class="top-search">
     <div class="top-search">
Line 161: Line 161:
</li>
</li>
<li class="current-menu-item"><a href="https://2012.igem.org/wiki/index.php?title=Team:Fudan_Lux/project_introduction">Project<span class="subheader">Cool</span></a><ul style="display: none; visibility: hidden; ">
<li class="current-menu-item"><a href="https://2012.igem.org/wiki/index.php?title=Team:Fudan_Lux/project_introduction">Project<span class="subheader">Cool</span></a><ul style="display: none; visibility: hidden; ">
-
<li><a href="https://2012.igem.org/wiki/index.php?title=Team:Fudan_Lux/project_introduction">Introduction</a></li>
+
<li><a href="https://2012.igem.org/wiki/index.php?title=Team:Fudan_Lux/project_introduction">Overview</a></li>
<li><a href="https://2012.igem.org/wiki/index.php?title=Team:Fudan_Lux/biowave">Project Biowave</a></li>
<li><a href="https://2012.igem.org/wiki/index.php?title=Team:Fudan_Lux/biowave">Project Biowave</a></li>
Line 225: Line 225:
<!-- meta -->
<!-- meta -->
<ul class="meta">
<ul class="meta">
-
<li><strong>Posted on</strong> Dec 27th 2011 </li>
+
<p>Multiple ultra-thin nanotubes formed between two Hela cells.</p>
-
<li><strong>By</strong> <a href="#">Ansimuz</a></li>
+
<li><strong>Posted on</strong> Sep 26th 2012 </li>
-
<li> <strong>Posted in</strong>  
+
<li><strong>By</strong> <a href="#">Tian Tian</a></li>  
<div class="meta-tags">
<div class="meta-tags">
-
<a href="#">Webdesign</a>
+
 
-
<a href="#">Code</a>
+
-
<a href="#">Photo</a>
+
</div>
</div>
</li>
</li>
Line 241: Line 239:
</div>
</div>
<div>
<div>
-
<p>Cellular communication is an old story. Multiple communication mechanisms, including  signal transduction, material transportation supported by multiple sub-cellular structures such as synaptic vesicle and junction complex, remain the hot spots of cell biology for many years. However, in addition to all the above-mentioned, are there any other communication mechanisms more intimate and straightforward? Take a look at the plasmodesma, which is a special cellular structure connecting neighboring plant cells. The two cells on each end of the plasmodesma share a certain amount of cytoplasm. Therefore, it serves as an important and unique pathway of communication in the plant kingdom. So is it possible for animal cells, which lack the rigid structure of cell wall, to form and maintain such a plasmodesma-like structure? May it succeed, what changes can be observed among the altered cells with this new type of communication? These are the questions that we tried to answer as we started out this project.
+
<p>Cellular communication is an old story. Multiple mechanisms for communication, including  signal transduction, material transportation supported by multiple sub-cellular structures such as synaptic vesicle and junction complex, have remained to be the hot spots of cell biology for many years. However, in addition to all the above-mentioned, are there any other communication mechanisms more intimate and straightforward? Take a look at the plasmodesma, which is a special cellular structure connecting neighboring plant cells. The two cells on each end of the plasmodesma share a certain amount of cytoplasm. Therefore, it serves as an important and unique pathway of communication in the plant kingdom. So is it possible for animal cells, which lack the rigid structure of the cell wall, to form and maintain such a plasmodesma-like structure? May it succeed, what changes can be observed among the altered cells with this new type of communication? These are the questions that we tried to answer as we started out this project.
</p>
</p>
 +
<p>On the way of realizing our original idea, we came across works done by experts much more sophisticated and experienced in this field. It turned out that the structure we wish to term "nanotube" here had already been mostly described as"Tunneling Nanotube(TNT)", whose first official documentation was made in cultured rat pheochromocytoma PC12 cells by Amin Rustom et. al back in 2004. However, due to its structural similarity to a regular cell protrusion, the significance of nanotube had never been fully discovered, let alone its vast potential in facilitating direct inter-cellular communication. Therefore, we Fudan-Lux set up an ambitious goal of achieving effective nanotube induction between Hela cells. In doing so, we wish to establish a brand-new type of life form among Hela cells as a syncytial entity, and further understand the importance of this intimate communication. </p>
 +
<p>However, as we Fudan-Lux understand it, communication is the basis of every advanced relationship. Events made possible by the establishment of communication between originally isolated parties might have even greater significance and application. Therefore, with induced nanotubes bridging neighboring cells, providing a super-highway for material transportation between connected cells, we came up with an idea of artificially introduce wild type E.coli into Hela cells. As these bacteria move within and between Hela cells seeking for their perfect habitat in a least energy-consuming fashion, we want to observe a distribution pattern that has the property of the least-entropy increase, which might have further application in our everyday life.
</div>
</div>
<div>
<div>
<h1><a name="Introduction">Introduction</a></h1>
<h1><a name="Introduction">Introduction</a></h1>
-
<p>The second project of Team Fudan-Lux is about constructing a brand-new biological model using a recently discovered cellular structure termed Tunneling Nanotubes(TNT) and bacteria containing the green fluorescence protein. By inducing and stabilizing TNTs between certain types of malignant tumor cells, a cellular network could be obtained. Then the bacteria containing GFP is introduced into the tumor cells by microinjection. By doing so, a new type of biological system is created. More importantly, what we want to study here, is the behavior of the injected bacteria within the tumor cells. Since TNTs formed between cells act as super highways for material transportation, bacteria thus can move from one cell to another via TNTs. Given the condition that bacteria would tend to choose the most suitable place for them to live in, in the least energy-consuming way, a distribution pattern thus can be obtained which have the characteristic of the least increase of entropy. By building such a model, we want to simulate certain types of problems in the real life that can’t be solved by simple computation, e.g. traffic jams between cities, and provide solutions to them.</p>
+
<p>The second project of Team Fudan-Lux is about constructing a brand-new biological model using a recently discovered cellular structure termed nanotubes and wild type E.coli K12 MG1655 containing the green fluorescence protein. By inducing and stabilizing nanotubes between cultured Hela cells, a cellular network could be obtained. Then the bacteria containing GFP is introduced into the tumor cells by modified electroporation. In doing so, a new type of biological system is created. More importantly, what we want to study here, is the behavior of the introduced bacteria within Hela cells. Since nanotubes formed between cells act as super highways for material transportation, bacteria thus can move from one cell to another via nanotubes. Given the condition that bacteria would tend to choose the most suitable place for them to live in, in the least energy-consuming way, a distribution pattern thus can be obtained, which have the characteristic of the least increase of entropy. By building such a model, we want to simulate certain types of problems in the real life that can’t be solved by simple computation, e.g. traffic jams between cities, and provide solutions to them.</p>
</div>
</div>
<div>
<div>
<h1><a name="model">Methods and Materials</a></h1>
<h1><a name="model">Methods and Materials</a></h1>
-
<img src="https://static.igem.org/mediawiki/igem.org/d/d4/F14024D5-5D49-4239-8915-7F154C3992F7.png" style="width:50%;float:right" class="myimg">
+
 
<p>Cell preparation</p>
<p>Cell preparation</p>
-
<p>We tried three approaches to induce the formation of nanotubes among Hela cells: Cholera toxin B induction, M-sec transfection and harsh environment induction (low pH, high glucose and low serum concentration), with the last one of highest efficiency.
+
<p>We tried three approaches to induce the formation of nanotubes among Hela cells: Cholera toxin B induction, M-sec transfection and harsh-environment simulation induction (low pH, high glucose and low serum concentration, referred as HES-induction in the following content), with the last one of highest efficiency.
   Hela cells with induced nanotubes and E.coli marked by Green Fluorescence Protein (GFP) were prepared for electroporation. After replacing culture medium with PBS, Hela cells were incubated with E.coli during exponential phase for 30 minutes. Before electroporation, the PBS was removed as thoroughly as possible in case of short circuit.</p>
   Hela cells with induced nanotubes and E.coli marked by Green Fluorescence Protein (GFP) were prepared for electroporation. After replacing culture medium with PBS, Hela cells were incubated with E.coli during exponential phase for 30 minutes. Before electroporation, the PBS was removed as thoroughly as possible in case of short circuit.</p>
<p>Electroporation</p>
<p>Electroporation</p>
 +
 +
<img src="https://static.igem.org/mediawiki/igem.org/d/d4/F14024D5-5D49-4239-8915-7F154C3992F7.png" style="width:100%;float:left" class="myimg">
<p>Illustrated schematically in Figure 1. After PBS was removed, two electrodes were inserted into the culture dish with their tips directly attached to the cell layer. Electrical pulses were delivered from a stimulator to a regular electric isolator, which was drived to output weak positive current (27–40 μA). The current flowed through the Hela cells from one electrode to the other. The waveform (square wave, 5Hz, 25ms) and amplitude of injected current were monitored via an oscilloscope to measure the voltage drop across a series resistor (75 KΩ), which was 2–3V.</p>
<p>Illustrated schematically in Figure 1. After PBS was removed, two electrodes were inserted into the culture dish with their tips directly attached to the cell layer. Electrical pulses were delivered from a stimulator to a regular electric isolator, which was drived to output weak positive current (27–40 μA). The current flowed through the Hela cells from one electrode to the other. The waveform (square wave, 5Hz, 25ms) and amplitude of injected current were monitored via an oscilloscope to measure the voltage drop across a series resistor (75 KΩ), which was 2–3V.</p>
Line 269: Line 271:
</div>
</div>
<div>
<div>
-
<h1><a name="lightsensor">Results</a></h1>
+
 
-
<p>Nanotube induction
+
-
(three pictures representing the different induction conditions & one showing the normal hela cells)
+
-
As it can be seen in these pictures, hela cells under induction would form a significantly higher amount of nanotubes in comparison with normal hela cells. Cells underwent harsh-environmental simulation induction displayed the most prompt and radical cellular structure changes. Two types of nanotubes have been observed: one was a wide (with a proximate diameter of ___) cell protrusion-like structure that reached out from one cell and directly touched another cell even across a rather long distance; another type of nanotube was comparatively much thiner, but in a rather great number. The latter type of nanotubes could be found at the end of the former ones, thereby further connecting distant cells; or right from the middle of the cell bodies linking several neighboring cells all at once. Both types were supported by F-actin and were non-adherent to the substratum.
+
-
Cultured cells stained with mitotracker after nanotubule induction and stabilization were then placed under microscope for observation. As it can been seen in this figure, mitochondria could travel from one cell to another via nanotube, demonstrating the property of material transportation of these induced nanotubes. Moreover, Ca2+ dye loading and two-photon Ca2+ imaging further confirmed the communication via Ca2+ flow between two connected cells.
+
-
With these experimental results available, we can basically confirm the cellular structures that we induced and stabilized here are those we anticipated.
+
-
When the harsh-environmental simulation induction prolonged, the cells with nanotubes underwent more radical changes of cellular structures. After 5 days’ induction, most living cells tended to distribute most of their plasma into wide and elongated nanotubes, resulting in an octopus-like shape of each single cell and a web-like system among the whole cell colony. Incubating these cells with normal culture media for one to two days, several originally distant cells connected merely by nanotubes moved towards each other and finally clustered together, forming one big syncytium that had interchangeable plasma and organelles via nanotubes. As cells of this syncytium divided, its state remained as the newborns, with nanotube-linked neighboring cells as well. Further Ca2+ dye loading and two-photon Ca2+ imaging demonstrated a synchronization of Ca2+ flow among each individual within this syncytium.
+
-
+
-
Electroporation& Microscopic imaging
+
-
Cultured Hela cells after electroporation were then placed under differential interference contrast(DIC) microscope for bacterial entrance verification. Wild type E.coli MG1655 expressing GFP could be seen inside the hela cells clearly. Most entered bacteria tended to cluster around the nucleus, a phenomena we reasoned that could optimize the bacterial distribution into daughter hela cells. Also, we observed a significantly higher GFP intensity given by the bacteria within hela cells, compared to bacteria stayed on the outside, which indicate that the plasma may be a more favorable place for this facultative anaerobe to live in. After executed harsh-environmental simulation induction among bacteria-breeding hela cells, symcytia similar to the former ones were obtained, with a typical spider web outlook. Visible E.coli MG1655 could be seen traveling from one hela cell to another via nanotubes, indicating a non-seletivity among the transporting cargo of these nanotubule highways. However, due to the viscosity of plasma and the retardance of the cytoskeleton, E.coli MG1655 within hela cells did not exhibit the mobility that we desired. In addition, the relationship between E.coli MG1655 and hela cells appeared to be rather intense as usually only one of these two parties can survive after a few days of incubation of this temperal man-made endosymbiotic system.
+
-
</p>
+
<p></p>
<p></p>
</div>
</div>
Line 285: Line 277:
<h1><a name="application">Future prospective</a></h1>
<h1><a name="application">Future prospective</a></h1>
<p> Nanotube part</p>
<p> Nanotube part</p>
-
<p>In this project, we successfully introduced a brand-new type of communication mechanism into cultured hela cells. As the first ones to establish such cellular structure in hela cells, we then were able to observe some of the inter-cellular communication that had never been documented in this cell line, including the complete exchange of organelles and cell-to-cell Ca2+ flow. But communication is just the first step. Changes made possible by such communication, whether between or within cells, we believe, are of even greater significance. So far we have already observed obvious cellular behavioral changes------single cells forming nanotubes in an attempt to reach out and touch some other cells. Based on what we’ve learned during the experiments, we have envisioned the following prospectives in further nanotubule research and application:</p>
+
<p>In this project, we successfully introduced a brand-new type of communication mechanism into cultured Hela cells. As the first ones to establish such cellular structure in Hela cells, we were then able to observe some of the inter-cellular communications that had never been documented in this cell line, including the complete exchange of organelles and cell-to-cell Ca2+ flow. But communication is merely the first step. Changes made possible by such communication, whether between or within cells, we believe, are of even greater significance. So far we have already observed obvious cellular behavioral changes------single cells forming nanotubes in an attempt to reach out and touch some other cells. Based on what we have learned during the experiments, we have envisioned the following prospectives in further nanotubular researches and applications:</p>
-
<p>1. From single cells to syncytium to multicellular organism?</p>  
+
<p>1. From single cell to syncytium, finally to multicellular organism?</p>  
-
<p>Combining the conditions under which we conducted the recovery experiment with the later visible cellular structral changes, we further came up with a thought of how such structure and communication mechanism functioned to boost the evolution from single cell to multi-cellular organisms. As we have already observed how the construction of the nanotubule network affect the organization among cell colonies------from single cells to a syncytial entity, which then move on to a multicellular colony among which a Ca2+ flow synchronization could be obtained.</p>
+
<p>Combining the conditions under which we conducted the recovery experiments with the later visible cellular structural changes, we further come up with a thought of how such structure and communication mechanism function to boost the evolution from a single cell to multi-cellular organisms. As we have already observed how the construction of the nanotubular network affect the organization within cell colonies------from single cells to a syncytial entity, which then move on to a multicellular colony in which a Ca2+ flow synchronization could be obtained.</p>
-
<p>2. A way to establish another version of a nerve system?</p>
+
<p>2. An approach to establish another version of a nervous system?</p>
-
<p>Some of the latest outcome of the harsh environment simulation induction were absolutely sensational, with some member of the syncytial entity developed the similar structure of neurons, connecting multiple parts of the entity via both types of nanotubes. Since calcium ion flow could be passed on to other cells via nanotubes, a process that may effectively alter the inner bio-chemical activities of the downstream cells, nanotubes thus could be considered as a counterpart of the axon of a neuron. With further modified induction solutions, we might be able to establish a multicellular structure that has certain features of the nerve tissue. </p>
+
<p>Some of the latest outcomes of the harsh environment simulation induction were absolutely sensational, with some members of the syncytial entity having developed the similar structure to neurons, connecting multiple parts of the entity via both types of nanotubes. Since Ca2+ flow can be passed on to other cells via nanotubes, a process that may effectively alter the inner bio-chemical activities of the downstream cells, nanotubes could be considered as a counterpart of the axon of a neuron. With further modified induction solutions, we might be able to establish a multicellular structure that has certain features of the nervous tissue. </p>
<p>3.Potential application in curing cancer?</p>
<p>3.Potential application in curing cancer?</p>
-
<p>As a communication channel which can facilitate the transportation of certain cellular contents, cells with large amount of nanotubes thus may also perform as a drug delivering system. By establishing cell lines that possess the property of high nanotubule formation and introducing them into tumor tissues, modified cells can form multiple connections with native cancer cells. If we introduce foreign genes encode for elements that might disturb the inner machinery of cancer cells, which could be produced by the modified cells and transported to the cancer cells via newly formed nanotubes. Such a new type of drug delivering system can thus achieve direct drug-delivery which could overcome some of the major drawbacks of present therapy for curing cancer.</p>
+
<p>As a communication channel which can facilitate the transportation of certain cellular contents, cells with large amounts of nanotubes may also perform as a drug delivering system. By cell lines that possess the property of high-level nanotubular formation and introducing them into tumor tissues, modified cells can form multiple connections with native cancer cells. If we introduce foreign genes into such modified cells, which encode for elements that might disturb the inner machinery of cancer cells and can be transported to them via newly formed nanotubes, a new type of direct drug-delivering system may be established. With the property of direct drug-delivery, it can overcome some of the major drawbacks of the present therapies for curing cancer.</p>
<p>Electroporation part</p>
<p>Electroporation part</p>
-
<p>1. Protein that enables synchronization among bacterial colony and facilitating bacterial distribution.</p>
+
<p>1. Protein that enables synchronization within bacteria colony and facilitating bacterial distribution?</p>
-
+
<p> Based on our experimental results, after entering Hela cells, wild type E.coli K12 MG1655 exhibited a certain degree of aggressiveness. That is to say, most cells invaded by bacteria died before we can observe obvious distribution pattern because of an uncontrolled bacterial proliferation. Hence in our later experiments, we decided to integrate the LOV protein that we made in Project Biowave, which had already been proven to have the ability to achieve synchronization among bacteria that express it, into our wild type E.coli K12 MG1655, in an attempt to synchronize invaded K12 MG1655, preventing it from over-reproduction within a rather short period of time.
<p>2. Endosymbiosis</p>
<p>2. Endosymbiosis</p>
-
<p>It could be considered as a bonus that with the successful introduction of bacteria into eukaryotic cells using modified electroporation, we actually became the very first ones to provide with a direct evidence in supporting the endosymbiosis hypothesis. Electrol shock and little eletro-conductive solution are two essential aspects ensuring bacterial entrance, an environmental condition that could be easily obtained back in those ancient days. Inspired by the experimental results, we were so shocked to realize that the process of establishing endosymbiosis might happen much more frequently than we once thought it would. Given a little charge of electricity and a conductive environment, bacteria move closely around eukaryotic cells could then enter the plasma.  </p>
+
<p>It can be considered as a bonus that with the successful introduction of bacteria into eukaryotic cells via modified electroporation, we actually become the very first ones to acquire a direct evidence in support of the endosymbiosis hypothesis. Electric shock and little conductive solution are two essential elements ensuring bacterial entrance, which can simulate an environmental condition that could be easily obtained back in ancient times. Inspired by the experimental results, we are so astonished to realize that the process of establishing endosymbiosis might happen much more frequently than we used to think it would. Given a weak positive current, bacteria can be driven to surround the eukaryotic cells and then enter the plasma via the instantaneous pores on the cell membranes.  </p>
<p></p>
<p></p>
 +
<h1><a name="model">References:</a></h1>
 +
<p> Rustom, A., R. Saffrich,.,H.H.Gerdes. 2004. Nanotubular highways for intercellular organelle transport. Science. 303:1007–1010. </p>
 +
<p> Koji Hase et al. M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex. 2009. Nat. Cell Biol. 11:1427–1432. </p>
 +
<p> Nagayama, S., Zeng, S., Xiong, W., Fletcher, M. L., Masurkar, A. V., Davis, D. J., Pieribone, V. A., Chen, W. R.. In vivo simultaneous tracing and Ca2+ imaging of local neuronal circuits. Neuron 53: 789-803, 2007. </p>
 +
<p> Ohki, K., Chung, S., Ch’ng, Y. H., Kara, P., Reid, R. C.. Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex. Nature 433: 597-603, 2005. </p>
 +
</div>
</div>
<!-- ENDS shadow -->
<!-- ENDS shadow -->
Line 324: Line 322:
<li class="cat-item"><a href="#Introduction" title="View all posts">Introduction</a></li>
<li class="cat-item"><a href="#Introduction" title="View all posts">Introduction</a></li>
<li class="cat-item"><a href="#model" title="View all posts">Methods and Material</a></li>
<li class="cat-item"><a href="#model" title="View all posts">Methods and Material</a></li>
-
<li class="cat-item"><a href="#lightsensor" title="View all posts">Results</a></li>
+
<li class="cat-item"><a href="https://2012.igem.org/Team:Fudan_Lux/result#10" title="View all posts">Results</a></li>
<li class="cat-item"><a href="#application" title="View all posts">Future Prospective</a></li>
<li class="cat-item"><a href="#application" title="View all posts">Future Prospective</a></li>
</ul>
</ul>
Line 343: Line 341:
<!-- ENDS MAIN -->
<!-- ENDS MAIN -->
-
<!-- Twitter -->
+
<!-- Twitter -->
<div id="twitter">
<div id="twitter">
-
<div class="wrapper">
+
-
<a href="#" id="prev-tweet"></a>
+
-
<a href="#" id="next-tweet"></a>
+
-
<img id="bird" src="img/bird.png" alt="Tweets" />
+
-
<div id="tweets">
+
-
<ul class="tweet_list"></ul>
+
-
</div>
+
-
</div>
+
</div>
</div>
<!-- ENDS Twitter -->
<!-- ENDS Twitter -->
Line 363: Line 354:
<!-- footer-cols -->
<!-- footer-cols -->
<ul id="footer-cols">
<ul id="footer-cols">
-
<li class="col">
+
-
<h6>Pages</h6>
+
-
<ul>
+
-
<li class="page_item"><a href="index.html">Home</a></li>
+
-
<li class="page_item"><a href="features.html">Features</a></li>
+
-
<li class="page_item"><a href="blog.html">Blog</a></li>
+
-
<li class="page_item"><a href="portfolio.html">Portfolio</a></li>
+
-
<li class="page_item"><a href="gallery.html">Gallery</a></li>
+
-
<li class="page_item"><a href="contact.html">Contact</a></li>
+
-
</ul>
+
-
</li>
+
<li class="col">
<li class="col">
-
<h6>Categories</h6>
+
<h6>About the Team</h6>
-
<ul>
+
He he he he!
-
<li><a href="#">Webdesign projects senectus</a></li>
+
-
<li><a href="#/">Wordpress projects senectus</a></li>
+
-
<li><a href="#">Vestibulum tortor quam</a></li>
+
-
<li><a href="#">Code projects amet quam egestas</a></li>
+
-
<li><a href="#">Web design projects senectus</a></li>
+
-
<li><a href="#/">Marketplace projects</a></li>
+
-
<li><a href="#">Writting projects senectus</a></li>
+
-
<li><a href="#">Drawings projects fames Aenean</a></li>
+
-
<li><a href="#/">Wordpress projects Aenean ultricies</a></li>
+
-
</ul>
+
-
</li>
+
-
<li class="col">
+
-
<h6>About the theme</h6>
+
-
Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo. Cursus faucibus, tortor neque egestas augue, eu vulputate magna eros.
+
</li>
</li>
Line 406: Line 374:
<!-- wrapper-bottom -->
<!-- wrapper-bottom -->
<div class="wrapper">
<div class="wrapper">
-
<div id="bottom-text">2011 Nova all rights reserved. <a href="http://www.luiszuno.com"> Luiszuno.com</a> </div>
+
<div id="bottom-text">All rights reserved. Tempalate Edit by Fudan-lux team <a href="http://www.luiszuno.com">Powered By Luiszuno.com</a> </div>
<!-- Social -->
<!-- Social -->
-
<ul class="social ">
+
-
<li><a href="http://www.facebook.com" class="poshytip  facebook" title="Become a fan"></a></li>
+
-
<li><a href="http://www.twitter.com" class="poshytip twitter" title="Follow our tweets"></a></li>
+
-
<li><a href="http://www.dribbble.com" class="poshytip dribbble" title="View our work"></a></li>
+
-
<li><a href="http://www.addthis.com" class="poshytip addthis" title="Tell everybody"></a></li>
+
-
<li><a href="http://www.vimeo.com" class="poshytip vimeo" title="View our videos"></a></li>
+
-
<li><a href="http://www.youtube.com" class="poshytip youtube" title="View our videos"></a></li>
+
-
</ul>
+
<!-- ENDS Social -->
<!-- ENDS Social -->
<div id="to-top" class="poshytip" title="To top"></div>
<div id="to-top" class="poshytip" title="To top"></div>
Line 422: Line 383:
</div>
</div>
<!-- ENDS Bottom -->
<!-- ENDS Bottom -->
-
+
 
</body>
</body>
</html>
</html>

Latest revision as of 03:36, 27 September 2012

NOVA

back to top
Project Bacto-trafficking Communication through nanotube!

Background

    Multiple ultra-thin nanotubes formed between two Hela cells.

  • Posted on Sep 26th 2012
  • By Tian Tian
Feature image

Cellular communication is an old story. Multiple mechanisms for communication, including signal transduction, material transportation supported by multiple sub-cellular structures such as synaptic vesicle and junction complex, have remained to be the hot spots of cell biology for many years. However, in addition to all the above-mentioned, are there any other communication mechanisms more intimate and straightforward? Take a look at the plasmodesma, which is a special cellular structure connecting neighboring plant cells. The two cells on each end of the plasmodesma share a certain amount of cytoplasm. Therefore, it serves as an important and unique pathway of communication in the plant kingdom. So is it possible for animal cells, which lack the rigid structure of the cell wall, to form and maintain such a plasmodesma-like structure? May it succeed, what changes can be observed among the altered cells with this new type of communication? These are the questions that we tried to answer as we started out this project.

On the way of realizing our original idea, we came across works done by experts much more sophisticated and experienced in this field. It turned out that the structure we wish to term "nanotube" here had already been mostly described as"Tunneling Nanotube(TNT)", whose first official documentation was made in cultured rat pheochromocytoma PC12 cells by Amin Rustom et. al back in 2004. However, due to its structural similarity to a regular cell protrusion, the significance of nanotube had never been fully discovered, let alone its vast potential in facilitating direct inter-cellular communication. Therefore, we Fudan-Lux set up an ambitious goal of achieving effective nanotube induction between Hela cells. In doing so, we wish to establish a brand-new type of life form among Hela cells as a syncytial entity, and further understand the importance of this intimate communication.

However, as we Fudan-Lux understand it, communication is the basis of every advanced relationship. Events made possible by the establishment of communication between originally isolated parties might have even greater significance and application. Therefore, with induced nanotubes bridging neighboring cells, providing a super-highway for material transportation between connected cells, we came up with an idea of artificially introduce wild type E.coli into Hela cells. As these bacteria move within and between Hela cells seeking for their perfect habitat in a least energy-consuming fashion, we want to observe a distribution pattern that has the property of the least-entropy increase, which might have further application in our everyday life.

Introduction

The second project of Team Fudan-Lux is about constructing a brand-new biological model using a recently discovered cellular structure termed nanotubes and wild type E.coli K12 MG1655 containing the green fluorescence protein. By inducing and stabilizing nanotubes between cultured Hela cells, a cellular network could be obtained. Then the bacteria containing GFP is introduced into the tumor cells by modified electroporation. In doing so, a new type of biological system is created. More importantly, what we want to study here, is the behavior of the introduced bacteria within Hela cells. Since nanotubes formed between cells act as super highways for material transportation, bacteria thus can move from one cell to another via nanotubes. Given the condition that bacteria would tend to choose the most suitable place for them to live in, in the least energy-consuming way, a distribution pattern thus can be obtained, which have the characteristic of the least increase of entropy. By building such a model, we want to simulate certain types of problems in the real life that can’t be solved by simple computation, e.g. traffic jams between cities, and provide solutions to them.

Methods and Materials

Cell preparation

We tried three approaches to induce the formation of nanotubes among Hela cells: Cholera toxin B induction, M-sec transfection and harsh-environment simulation induction (low pH, high glucose and low serum concentration, referred as HES-induction in the following content), with the last one of highest efficiency. Hela cells with induced nanotubes and E.coli marked by Green Fluorescence Protein (GFP) were prepared for electroporation. After replacing culture medium with PBS, Hela cells were incubated with E.coli during exponential phase for 30 minutes. Before electroporation, the PBS was removed as thoroughly as possible in case of short circuit.

Electroporation

Illustrated schematically in Figure 1. After PBS was removed, two electrodes were inserted into the culture dish with their tips directly attached to the cell layer. Electrical pulses were delivered from a stimulator to a regular electric isolator, which was drived to output weak positive current (27–40 μA). The current flowed through the Hela cells from one electrode to the other. The waveform (square wave, 5Hz, 25ms) and amplitude of injected current were monitored via an oscilloscope to measure the voltage drop across a series resistor (75 KΩ), which was 2–3V.

Dye loading and two-photon Ca2+ imaging

We loaded nanotube-linked Hela cells with a Ca2+-sensitive dye and observed the Ca2+ flow through two-photon microscope. A total of 1μL Fluo-2 was diluted 1:1000 with 1× PBS. A culture dish of Hela cells was incubated with this solution for 35 minutes at 37℃ to full loading. After confirming loading, the Fluo-2 dye was completely washed off by PBS, repeating 3 to 4 times. Two-photon imaging of changes in Ca2+ fluorescence in Hela cells was monitered with a costum-built microscope coupled with a Mai Tai mode-locked Ti:sapphire laser (730–740 nm).

Future prospective

Nanotube part

In this project, we successfully introduced a brand-new type of communication mechanism into cultured Hela cells. As the first ones to establish such cellular structure in Hela cells, we were then able to observe some of the inter-cellular communications that had never been documented in this cell line, including the complete exchange of organelles and cell-to-cell Ca2+ flow. But communication is merely the first step. Changes made possible by such communication, whether between or within cells, we believe, are of even greater significance. So far we have already observed obvious cellular behavioral changes------single cells forming nanotubes in an attempt to reach out and touch some other cells. Based on what we have learned during the experiments, we have envisioned the following prospectives in further nanotubular researches and applications:

1. From single cell to syncytium, finally to multicellular organism?

Combining the conditions under which we conducted the recovery experiments with the later visible cellular structural changes, we further come up with a thought of how such structure and communication mechanism function to boost the evolution from a single cell to multi-cellular organisms. As we have already observed how the construction of the nanotubular network affect the organization within cell colonies------from single cells to a syncytial entity, which then move on to a multicellular colony in which a Ca2+ flow synchronization could be obtained.

2. An approach to establish another version of a nervous system?

Some of the latest outcomes of the harsh environment simulation induction were absolutely sensational, with some members of the syncytial entity having developed the similar structure to neurons, connecting multiple parts of the entity via both types of nanotubes. Since Ca2+ flow can be passed on to other cells via nanotubes, a process that may effectively alter the inner bio-chemical activities of the downstream cells, nanotubes could be considered as a counterpart of the axon of a neuron. With further modified induction solutions, we might be able to establish a multicellular structure that has certain features of the nervous tissue.

3.Potential application in curing cancer?

As a communication channel which can facilitate the transportation of certain cellular contents, cells with large amounts of nanotubes may also perform as a drug delivering system. By cell lines that possess the property of high-level nanotubular formation and introducing them into tumor tissues, modified cells can form multiple connections with native cancer cells. If we introduce foreign genes into such modified cells, which encode for elements that might disturb the inner machinery of cancer cells and can be transported to them via newly formed nanotubes, a new type of direct drug-delivering system may be established. With the property of direct drug-delivery, it can overcome some of the major drawbacks of the present therapies for curing cancer.

Electroporation part

1. Protein that enables synchronization within bacteria colony and facilitating bacterial distribution?

Based on our experimental results, after entering Hela cells, wild type E.coli K12 MG1655 exhibited a certain degree of aggressiveness. That is to say, most cells invaded by bacteria died before we can observe obvious distribution pattern because of an uncontrolled bacterial proliferation. Hence in our later experiments, we decided to integrate the LOV protein that we made in Project Biowave, which had already been proven to have the ability to achieve synchronization among bacteria that express it, into our wild type E.coli K12 MG1655, in an attempt to synchronize invaded K12 MG1655, preventing it from over-reproduction within a rather short period of time.

2. Endosymbiosis

It can be considered as a bonus that with the successful introduction of bacteria into eukaryotic cells via modified electroporation, we actually become the very first ones to acquire a direct evidence in support of the endosymbiosis hypothesis. Electric shock and little conductive solution are two essential elements ensuring bacterial entrance, which can simulate an environmental condition that could be easily obtained back in ancient times. Inspired by the experimental results, we are so astonished to realize that the process of establishing endosymbiosis might happen much more frequently than we used to think it would. Given a weak positive current, bacteria can be driven to surround the eukaryotic cells and then enter the plasma via the instantaneous pores on the cell membranes.

References:

Rustom, A., R. Saffrich,.,H.H.Gerdes. 2004. Nanotubular highways for intercellular organelle transport. Science. 303:1007–1010.

Koji Hase et al. M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex. 2009. Nat. Cell Biol. 11:1427–1432.

Nagayama, S., Zeng, S., Xiong, W., Fletcher, M. L., Masurkar, A. V., Davis, D. J., Pieribone, V. A., Chen, W. R.. In vivo simultaneous tracing and Ca2+ imaging of local neuronal circuits. Neuron 53: 789-803, 2007.

Ohki, K., Chung, S., Ch’ng, Y. H., Kara, P., Reid, R. C.. Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex. Nature 433: 597-603, 2005.

All rights reserved. Tempalate Edit by Fudan-lux team Powered By Luiszuno.com