Team:University College London/Module 4/Results

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(Characterisation of Starvation Promoter BBa_K118011)
(Characterisation of Starvation Promoter BBa_K118011)
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Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%).  
Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%).  
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We carried out further characterization of the pcstA fused with GFP (BBa_K200018) built by Imperial College's 2009 iGem team. Fluorescence was measured from overnight cultures and data collected shows significant higher expression for cultures with 0%, 0.2%, 0.5% glucose concentration, this essay also shows how glucose represses cstA promoter.
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[[File:UniversityCollegeLondon_Buoyancy_K200018.jpg]]
[[File:UniversityCollegeLondon_Buoyancy_K200018.jpg]]

Revision as of 13:23, 26 September 2012

Module 4: Buoyancy

Description | Design | Construction | Characterisation | Modelling | Results | Conclusions

Characterisation of Starvation Promoter BBa_K118011

Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%).


UniversityCollegeLondon Buoyancy K200018.jpg We carried out further characterization of the pcstA fused with GFP (BBa_K200018) built by Imperial College's 2009 iGem team. Fluorescence was measured from overnight cultures and data collected shows significant higher expression for cultures with 0%, 0.2%, 0.5% glucose concentration, this essay also shows how glucose represses cstA promoter.


UniversityCollegeLondon Buoyancy K729008.jpg UniversityCollegeLondon Buoyancy Comparison.jpg