"
Team:TMU-Tokyo/Project device1
From 2012.igem.org
| Line 29: | Line 29: | ||
<p class="description"> | <p class="description"> | ||
| - | In <i>frmRAB</i> operon, which origin is chromosome of <i>Escherichia coli</i>, <i>frmAB</i> is normally repressed by frmR. But, when formaldehyde exists, <i>frmR</i> loses its inhibitory and <i>frmAB</i> is expressed. | + | In <i>frmRAB</i> operon, which origin is chromosome of <i>Escherichia coli</i>, <i>frmAB</i> is normally repressed by frmR. But, when formaldehyde exists, <i>frmR</i> loses its inhibitory and <i>frmAB</i> is expressed. |
<Br> | <Br> | ||
This device is composed of <i>frmR</i> gene, its promoter and GFP gene.</p> | This device is composed of <i>frmR</i> gene, its promoter and GFP gene.</p> | ||
| Line 35: | Line 35: | ||
<center><Img Src="https://static.igem.org/mediawiki/2012/3/3a/Device1.png"></center><Br> | <center><Img Src="https://static.igem.org/mediawiki/2012/3/3a/Device1.png"></center><Br> | ||
<p class="description"> | <p class="description"> | ||
| - | By using this device, we can visualize formaldehyde with GFP. | + | By using this device, we can visualize formaldehyde with GFP. This visualization system enable to make us know how much of the formaldehyde concentration there is.<Br> |
| + | |||
| - | |||
| - | |||
<Br> | <Br> | ||
| + | </p> | ||
<Br> | <Br> | ||
| + | |||
| Line 47: | Line 48: | ||
<p class="description2"> | <p class="description2"> | ||
| - | + | <B>■The detail of <i>frmR</i> effect</B> | |
| + | <Br><Strong>Christopher D. Herring and Frederick R. Blattner.</Strong> 2004. Global Transcriptional Effects of a Suppressor tRNA and the Inactivation of the Regulator <i>frmR</i> [<A Href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC522192/">PubMed</A>]<Br> | ||
<Br> | <Br> | ||
| Line 55: | Line 57: | ||
| + | </div> | ||
| + | <div class="grid_1"> </div> | ||
| + | |||
| + | <div class="clear"></div> | ||
| - | + | ||
| + | |||
| + | |||
| + | <div class="container_12"> | ||
<div class="grid_1"> </div> | <div class="grid_1"> </div> | ||
| + | <div class="grid_10"> | ||
| + | |||
| + | |||
| + | <Br><p class="description"><Strong>Details of <i>frmR</i> repression</Strong><Br> | ||
| + | <Hr><Br> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="grid_1"> </div> | ||
| + | |||
<div class="clear"></div> | <div class="clear"></div> | ||
| + | |||
| + | |||
</Html> | </Html> | ||
Revision as of 19:15, 13 September 2012
Device1;
Sensing formaldehyde and then producing GFP
In frmRAB operon, which origin is chromosome of Escherichia coli, frmAB is normally repressed by frmR. But, when formaldehyde exists, frmR loses its inhibitory and frmAB is expressed.
This device is composed of frmR gene, its promoter and GFP gene.
By using this device, we can visualize formaldehyde with GFP. This visualization system enable to make us know how much of the formaldehyde concentration there is.
■The detail of frmR effect
Christopher D. Herring and Frederick R. Blattner. 2004. Global Transcriptional Effects of a Suppressor tRNA and the Inactivation of the Regulator frmR [PubMed]
fig1. a) When formaldehyde doesn't exist, frmR represses upstream promoter. In this state, transcription is stopped and GFP isn't produced. b) When formaldehyde exists, it makes frmR lose activity. Then promoter is released from frmR's repress and begins to produce GFP.
Details of frmR repression
