Team:TMU-Tokyo/Project abstract

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Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.<Br>
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We planed to create E.coli which removes formaldehyde, named <Strong>Chef Ant E.coli</Strong>. It is appended 3 devices and gets removal ability of formaldehyde.<Br>
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<Img Src="https://static.igem.org/mediawiki/2012/7/7d/TMU-Tokyo_project_fig1.png"><Br>
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The pathway of formaldehyde remove is done through 2 stages. First, formaldehyde dehydrogenase derived from <i>Pseudomonas putida</i> (PFDH) changes formaldehyde to formate. Subsequently, formate dehydrogenase derived from <i>Methylobacterium extorquens</i> (FDH4) changes formate to water and carbon dioxide.<Br>
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In parallel with this dehydrohenation, formaldehyde content is measured with GFP. So <b>Chef Ant E. coli</b> enables to visualize formaldehyde decomposing process.<Br>
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Following is device details.
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We planed to create E.coli which removes such a hazardous material, named <Strong>Chef Ant E.coli</Strong>. It is appended 3 devices and gets removal ability of formaldehyde.<Br>
 
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<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device1"><b>Device1</b></A>; Visualize formaldehyde with GFP.<Br>
<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device1"><b>Device1</b></A>; Visualize formaldehyde with GFP.<Br>
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<B>■Tonegawa's formaldehyde levels spike</B><Br>
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― <A Href="http://www.yomiuri.co.jp/dy/national/T120521004335.htm">DAILY YOMIURI ONLINE</A><Br><Br><Br><Br><Br><Br>
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<B>■fig.1</B><Br>
<B>■fig.1</B><Br>

Revision as of 15:15, 23 September 2012

 


Abstract


 
 

We planed to create E.coli which removes formaldehyde, named Chef Ant E.coli. It is appended 3 devices and gets removal ability of formaldehyde.


The pathway of formaldehyde remove is done through 2 stages. First, formaldehyde dehydrogenase derived from Pseudomonas putida (PFDH) changes formaldehyde to formate. Subsequently, formate dehydrogenase derived from Methylobacterium extorquens (FDH4) changes formate to water and carbon dioxide.

In parallel with this dehydrohenation, formaldehyde content is measured with GFP. So Chef Ant E. coli enables to visualize formaldehyde decomposing process.


Following is device details.

Device1; Visualize formaldehyde with GFP.
Device2; Dehydrogenize formaldehyde to formate.
Device3; Dehydrogenize formate to carbon dioxide and water.

Details of our devices is available each device page.









■fig.1
This is the pathway of formaldehyde visualization and removal.

 

Project Top Abstract Device1 Device2 Device3

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