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Team:TMU-Tokyo/Notebook/Assay 3 Protocol and Result
From 2012.igem.org
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<b>■Assay3 Result</b><Br> | <b>■Assay3 Result</b><Br> | ||
<Br> | <Br> | ||
| - | + | <b>BBa_K749015</b> | |
| + | <Br> | ||
30min 50μl 229371.2 80.88<Br> | 30min 50μl 229371.2 80.88<Br> | ||
10min 20μl 248294.2 83.9823<Br> | 10min 20μl 248294.2 83.9823<Br> | ||
10min No enzym 243418.6 84.37<Br> | 10min No enzym 243418.6 84.37<Br> | ||
| - | + | <b>WT</b><Br> | |
| + | 10min 20μl 232791.6 83.691<Br> | ||
</p> | </p> | ||
Revision as of 23:41, 26 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Assay 3
■Assay3 Protocol
1The cells were cultured at 30 ℃ for 18 hours in medium
2Centrifuged 10000 × g 5min
3Remove the supernatant
4Wash the fungus
E. coli disrupted by sonicator in buffer of 50mM Tris-HCl pH7.2 .
Centrifuged 10000 × g at 4 ℃ so that the enzyme is not deactivated, the supernatant was harvested as crude enzyme solution (If you want to save is stored at 4 ℃ in a cold room)
■Assay3 Result
BBa_K749015
30min 50μl 229371.2 80.88
10min 20μl 248294.2 83.9823
10min No enzym 243418.6 84.37
WT
10min 20μl 232791.6 83.691
