Team:TMU-Tokyo/Notebook/Assay

From 2012.igem.org

Revision as of 19:44, 25 September 2012 by T.acts (Talk | contribs)

 




■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)


■Protocols
Plasmid DNA Pure
Genome DNA Purification Restruction Purification
DNA Fragment Ligation
Transformation
Electrophoresis
LB Medium


■Assay
Device1 Assay
Device2 Assay
Device3 Assay




Assay



■Assay1(Device1)


■Assay2(Device2)


■Assay3(Device3)
FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺ at 340nm.