"
Team:Paris Bettencourt/Semantic containment
From 2012.igem.org
(→Overview) |
|||
| Line 3: | Line 3: | ||
<div id="grouptitle">Semantic containment</div> | <div id="grouptitle">Semantic containment</div> | ||
==Overview== | ==Overview== | ||
| - | We | + | We want to prevent our genetic construct from conferring an advantage to other organisms. Horizontal gene transfer (HGT) can be performed either by conjugation, transduction, or transformation. As these processes involve two parties, our genetically modified bacteria and some wild type population, and as we will not modify wild type populations, we cannot assume that HGT is fully avoidable. Semantic containment [1] means that our bacteria won't be able to "speak" with other organisms, since they don't speak the same "language", the language being DNA. Our system will read the stop codon TAG as the amino-acid serine. It means that in our bacteria the stop codon will be translated into a serine, whereas in wild type bacteria this protein will be truncated and will not confer an advantage to these cells. |
| - | + | ||
==Objectives== | ==Objectives== | ||
Revision as of 14:23, 3 September 2012
- Main
- Team
- Project
- Achievements
- Human Practice
- Safety
- Notebook
- Attributions
Overview
Delay system
Semantic containment
Restriction enzyme system
MAGE
Suicide system
Encapsulation
Synthetic import domain
Safety Questions
Safety AssessmentContents |
Overview
We want to prevent our genetic construct from conferring an advantage to other organisms. Horizontal gene transfer (HGT) can be performed either by conjugation, transduction, or transformation. As these processes involve two parties, our genetically modified bacteria and some wild type population, and as we will not modify wild type populations, we cannot assume that HGT is fully avoidable. Semantic containment [1] means that our bacteria won't be able to "speak" with other organisms, since they don't speak the same "language", the language being DNA. Our system will read the stop codon TAG as the amino-acid serine. It means that in our bacteria the stop codon will be translated into a serine, whereas in wild type bacteria this protein will be truncated and will not confer an advantage to these cells.
Objectives
Write here the objectives of your project
Design
Present the design of your system, both in a written form, and a schematic one.
Experiments and results
Characterisation of X
Experimental setup
Describe the experiment
Results
Present your results
Testing of the system
Experimental setup
Describe the experiment
Results
Present your results
