Team:HokkaidoU Japan/Notebook/plastic protocols
From 2012.igem.org
Polymer extraction and purification
1. Move dried up bacteria into test tube.
- 2. Break up them to separate and add 10ml chloroform.
- 3. Incubate for 48hour at 60C.
- 4. Make it filtered through PTFE and move it into another test tube.
- 5. Volatilize organic solvent by exposing air and separate polymer.
- 6. Add 5ml hexane and voltex for a minute. After centrifuging (1,500rpm, 10min), remove the clear layer.
- 7. Volatilize chloroform by exposing air again.
- 8. Add 5ml MtOH, voltex for a minute, centrifuge (1,500rpm, 10min), and remove the clear layer.