Team:HokkaidoU Japan/Notebook/plastic Week 8
From 2012.igem.org
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- | A digestion to divide PhaC and pSB1C3 with XbaI and SpeI. </ br> | + | A digestion to divide PhaC and pSB1C3 with XbaI and SpeI. </br> |
{|class="hokkaidou-table-digestion" | {|class="hokkaidou-table-digestion" |
Revision as of 07:47, 29 August 2012
Contents |
August 20th
Digestion
A digestion to divide PhaC and pSB1C3 with XbaI and SpeI. </br>
DNA solution PhaC | 12 ul |
XbaI | 1 ul |
SpeI | 1 ul |
10xM buffer | 2 ul |
DW | 4 ul |
Total | 20 ul |
A digestion to divide PhaA and XbaI site and SpeI site.</ br>
DNA solution PhaA | 7 ul |
XbaI | 1 ul |
SpeI | 1 ul |
10xM buffer | 2 ul |
DW | 9 ul |
Total | 20 ul |
A digestion to divide PhaB and XbaI site and SpeI site.</ br>
DNA solution PhaB | 7 ul |
XbaI | 1 ul |
SpeI | 1 ul |
10xM buffer | 2 ul |
DW | 9 ul |
Total | 20 ul |
August 21th
August 24th
Colony PCR
August 25th
Colony PCR
August 26th
Mini prep of DNA