Team:HokkaidoU Japan/Notebook/plastic Week 8

From 2012.igem.org

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(August 20th)
(Gel extraction)
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Gel ectraction of PhaA, PhaB, pSB1C3 digestion result.</ br>
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Gel ectraction of PhaA, PhaB, pSB1C3 digestion result.
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We used FastGene Gel&PCR extraction kit(NipponGenetics)and got 50 ul of DNA solution.</ br>
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We used FastGene Gel&PCR extraction kit(NipponGenetics)and got 50 ul of DNA solution.
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Revision as of 14:29, 26 August 2012

Contents

August 20th

Digestion

A digestion to divide PhaC and pSB1C3 with XbaI and SpeI. </ br>

DNA solution PhaC 12 ul
XbaI 1 ul
SpeI 1 ul
10xM buffer 2 ul
DW 4 ul
Total 20 ul

A digestion to divide PhaA and XbaI site and SpeI site.</ br>

DNA solution PhaA 7 ul
XbaI 1 ul
SpeI 1 ul
10xM buffer 2 ul
DW 9 ul
Total 20 ul


A digestion to divide PhaB and XbaI site and SpeI site.</ br>

DNA solution PhaB 7 ul
XbaI 1 ul
SpeI 1 ul
10xM buffer 2 ul
DW 9 ul
Total 20 ul


August 21th

Elecrophoresis

Gel extraction

Gel ectraction of PhaA, PhaB, pSB1C3 digestion result. We used FastGene Gel&PCR extraction kit(NipponGenetics)and got 50 ul of DNA solution.

August 22th

Ethanol precipitation


Ligation

Transformation


August 23th

Colony PCR

August 24th

Colony PCR


August 25th

Colony PCR

August 26th

Mini prep of DNA