Team:HokkaidoU Japan/Notebook/plastic Week 8

From 2012.igem.org

(Difference between revisions)
(→August 20th)
(→August 20th)
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==August  20th==
==August  20th==
<div>
<div>
-
 
+
==Digestion==
<p>
<p>
-
A digestion to divide PhaC and pSB1C3 using .
+
A digestion to divide PhaC and pSB1C3 with XbaI and SpeI. </ br>
{|class="hokkaidou-table-digestion"
{|class="hokkaidou-table-digestion"
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   |20 ul
   |20 ul
   |}
   |}
 +
 +
A digestion to divide PhaA and XbaI site and SpeI site.</ br>
 +
{|class="hokkaidou-table-digestion"
 +
  |-
 +
  |DNA solution PhaA
 +
  |7 ul
 +
  |-
 +
  |XbaI
 +
  |1 ul
 +
  |-
 +
  |SpeI
 +
  |1 ul
 +
  |-
 +
  |10xM buffer
 +
  |2 ul
 +
  |-
 +
  |DW
 +
  |9 ul
 +
  |-
 +
  |Total
 +
  |20 ul
 +
  |}
 +
 +
 +
A digestion to divide PhaB and XbaI site and SpeI site.</ br>
 +
{|class="hokkaidou-table-digestion"
 +
  |-
 +
  |DNA solution PhaA
 +
  |7 ul
 +
  |-
 +
  |XbaI
 +
  |1 ul
 +
  |-
 +
  |SpeI
 +
  |1 ul
 +
  |-
 +
  |10xM buffer
 +
  |2 ul
 +
  |-
 +
  |DW
 +
  |9 ul
 +
  |-
 +
  |Total
 +
  |20 ul
 +
  |}
 +

Revision as of 14:13, 26 August 2012

Contents

August 20th

Digestion

A digestion to divide PhaC and pSB1C3 with XbaI and SpeI. </ br>

DNA solution PhaC ( 257ng/ul) 12 ul
XbaI 1 ul
SpeI 1 ul
10xM buffer 2 ul
DW 4 ul
Total 20 ul

A digestion to divide PhaA and XbaI site and SpeI site.</ br>

DNA solution PhaA 7 ul
XbaI 1 ul
SpeI 1 ul
10xM buffer 2 ul
DW 9 ul
Total 20 ul


A digestion to divide PhaB and XbaI site and SpeI site.</ br>

DNA solution PhaA 7 ul
XbaI 1 ul
SpeI 1 ul
10xM buffer 2 ul
DW 9 ul
Total 20 ul


August 21th

Colony PCR

August 22th

Colony PCR


August 23th

Colony PCR

August 24th

Colony PCR


August 25th

Colony PCR

August 26th

Mini prep of DNA





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