Team:HokkaidoU Japan/Notebook/plastic Week 6

From 2012.igem.org

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(DNA sequencing)
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==DNA sequencing==
==DNA sequencing==
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We took the sequence of BBa_K342001(PhaC).<br />
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BBa_K342001(PhaC) was seqenced.<br />

Revision as of 06:29, 30 August 2012

Contents

August 6th

polymer extract

@Taguchi lab

  1. sample1 JM109 culture from July18th 1.4643g , 0.0054 (tube+polymer , polymer)
  2. sample2 JM109 culture from July18th 1.4642g , 0.0040
  3. sample3 DH5a 1.4666g , 0.0056

preculture

Inoculated from three different plates(Name it 1,2,3)
Cultured in media of LB 150microL+Amp1.5microL
30℃ 20:00-7th10:00

August 7th

liquid culture

We made 8sapmles to see if Panthotenic acid is nessesary for plastic growth.
Panthotenic acid (+)1.5ml 4samples
Panthotenic acid (+)0.7ml 2samples
Panthotenic acid (-)0.7ml 2samples
Started liquid culture from Aug 7th 11:00 until Aug 9th 09:50.</ br>

August 9th

@Taguchi lab

Harvest

We did the protocol of Harvest on 8samples.

August 10th

miniprep

We did the method of mini-prep for BBa_K342001(PhaC). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions.

August 11th

DNA sequencing

BBa_K342001(PhaC) was seqenced.