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Team:HokkaidoU Japan/Notebook/plastic Week 11
From 2012.igem.org
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==PCR== | ==PCR== | ||
<p> | <p> | ||
| - | PhaA was multiplied from pGEM by PCR. | + | PhaA was multiplied from pGEM with XbaI and SpeI restriction sites by PCR. |
</p> | </p> | ||
| + | ==Digestion== | ||
| + | <p> | ||
| + | PhaA was digested with XbaI and SpeI. RBS on pSB1A2 was digested with SpeI. | ||
| + | </p> | ||
| + | ==Gel extraction== | ||
| + | <p> | ||
| + | We confirmed succession of digestion by electrophoresis.<br/> | ||
| + | And then DNA were extracted from TBE gel. | ||
| + | </p> | ||
| + | |||
| + | =ligation= | ||
| + | <p> | ||
| + | RBS on pSB1A2 was ligated with PhaA and PhaB. | ||
| + | </p> | ||
| + | <div/><div/> | ||
<!-- DO NOT EDIT UNDER THIS LINE @iTakeshi --> | <!-- DO NOT EDIT UNDER THIS LINE @iTakeshi --> | ||
Revision as of 10:01, 10 September 2012
Contents |
September 10th
PCR
PhaA was multiplied from pGEM with XbaI and SpeI restriction sites by PCR.
Digestion
PhaA was digested with XbaI and SpeI. RBS on pSB1A2 was digested with SpeI.
Gel extraction
We confirmed succession of digestion by electrophoresis.
And then DNA were extracted from TBE gel.
ligation
RBS on pSB1A2 was ligated with PhaA and PhaB.
<div/><div/>
