Team:HokkaidoU Japan/Notebook/aggregation Week 4

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July 16th

Mini-prep

mini-prep for Ag43(7/17 cultivated colony resuspended and 7/20 cultivated colony resuspended). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50ul of DNA solutions.

Electrophoresis

Electrophoresis for Ag43(mini-preped above) and Ag43 digestion results(digested with EcoRI and SpeI)

[[image:|thumb|Mini-prep result]]

[[image:|thumb|Digestion result]]