Team:HokkaidoU Japan/Notebook/aggregation Week 4
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==Mini-prep== | ==Mini-prep== | ||
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mini-prep for Ag43(7/17 cultivated colony resuspended and 7/20 cultivated colony resuspended). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50ul of DNA solutions. | mini-prep for Ag43(7/17 cultivated colony resuspended and 7/20 cultivated colony resuspended). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50ul of DNA solutions. | ||
+ | </p> | ||
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+ | ==Electrophoresis== | ||
+ | Electrophoresis for Ag43(mini-preped above) and Ag43 digestion results(digested with EcoRI and SpeI) | ||
+ | |||
+ | [[image:|thumb|Mini-prep result]] | ||
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+ | [[image:|thumb|Digestion result]] | ||
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</div> | </div> | ||
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Revision as of 04:08, 24 July 2012
July 16th
Mini-prep
mini-prep for Ag43(7/17 cultivated colony resuspended and 7/20 cultivated colony resuspended). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50ul of DNA solutions.
Electrophoresis
Electrophoresis for Ag43(mini-preped above) and Ag43 digestion results(digested with EcoRI and SpeI)
[[image:|thumb|Mini-prep result]]
[[image:|thumb|Digestion result]]